Journal Article

Impact of Salt and Nutrient Content on Biofilm Formation by Vibrio fischeri

DeLoney-Marino, Cindy R., Ondrey, Jakob M., Marsden, Anne E., Visick, Karen L., Grudzinski, Kevin

2017

Overview

Vibrio fischeri, a marine bacterium and symbiont of the Hawaiian bobtail squid Euprymna scolopes, depends on biofilm formation for successful colonization of the squid's symbiotic light organ. Here, we investigated if culture conditions, such as nutrient and salt availability, affect biofilm formation by V. fischeri by testing the formation of wrinkled colonies on solid media. We found that V. fischeri forms colonies with more substantial wrinkling when grown on the nutrient-dense LBS medium containing NaCl relative to those formed on the more nutrient-poor, seawater-salt containing SWT medium. The presence of both tryptone and yeast extract was necessary for the production of \"normal\" wrinkled colonies; when grown on tryptone alone, the colonies displayed a divoting phenotype and were attached to the agar surface. We also found that the type and concentration of specific seawater salts influenced the timing of biofilm formation. Of the conditions assayed, wrinkled colony formation occurred earliest in LBS(-Tris) media containing 425 mM NaCl, 35 mM MgSO4, and 5 mM CaCl2. Pellicle formation, another measure of biofilm development, was also enhanced in these growth conditions. Therefore, both nutrient and salt availability contribute to V. fischeri biofilm formation. While growth was unaffected, these optimized conditions resulted in increased syp locus expression as measured by a PsypA-lacZ transcriptional reporter. We anticipate these studies will help us understand how the natural environment of V. fischeri affects its ability to form biofilms and, ultimately, colonize E. scolopes.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Agar / Aliivibrio fischeri - drug effects / Aliivibrio fischeri - growth & development / Aquatic life / Bacterial proteins / Biofilms / Biofilms - drug effects / Biofilms - growth & development / Biology and Life Sciences / Calcium chloride / Colonies / Colonization / Culture Media / E coli / Earth Sciences / Escherichia coli / Euprymna scolopes / Genes / Growth conditions / Immunology / Influence / Laboratories / Light / Media (culture) / Microbial mats / Motility / Natural environment / Nutrient availability / Nutrient content / Nutrients / Pellicle / Physiological aspects / Plasmids / Research and Analysis Methods / Salts / Scolopes / Seawater / Sensors / Sodium chloride / Sodium Chloride - pharmacology / Sodium in the body / Squid / Transcription / Vibrio / Vibrio cholerae / Vibrio fischeri / Vibrio vulnificus / Water-borne diseases / Waterborne diseases / Yeast