Journal Article

Selective production of two diastereomers of disaccharide sugar alcohol, mannosylerythritol by Pseudozyma yeasts

Yoshikawa, Jun, Fukuoka, Tokuma, Kitamoto, Dai, Imura, Tomohiro, Konishi, Masaaki, Morita, Tomotake, Kakugawa, Koji

2014

Overview

Mannosylerythritol (ME) is the hydrophilic backbone of mannosylerythritol lipids as the most promising biosurfactants produced by different Pseudozyma yeasts, and has been receiving attention as a new sugar alcohol. Different Pseudozyma yeasts were examined for the sugar alcohol production using glucose as the sole carbon source. P. hubeiensis KM-59 highly produced a conventional type of ME, i.e., 4-O-β-D-mannopyranosyl-D-erythritol (4-ME). Interestingly, P. tsukubaensis KM-160 produced a diastereomer of 4-ME, i.e., 1-O-β-D-mannopyranosyl-D-erythritol (1-ME). In shake flask culture with 200 g/l of glucose, strain KM-59 produced 4-ME at a yield of 33.2 g/l (2.2 g/l/day of the productivity), while strain KM-160 produced 1-ME at 30.0 g/l (2.0 g/l/day). Moreover, the two strains were found to produce ME from glycerol; the maximum yields of 4-ME and 1-ME from 200 g/l of glycerol were 16.1 g/l (1.1 g/l/day) and 15.8 g/l (1.1 g/l/day), respectively. The production of 1-ME as the new diastereomer was further investigated in fed batch culture using a 5-l jar-fermenter. Compared to the flask culture, strain KM-160 gave three times higher productivity of 1-ME at 38.0 g/l (6.3 g/l/day) from glucose and at 31.1 g/l (3.5 g/l/day) from glycerol, respectively. This is the first report on the selective production of two diastereomers of ME, and should thus facilitate the functional development and application of the disaccharide sugar alcohol in the food and relative industries.

• Publisher: Springer-Verlag,Springer Science and Business Media LLC,Springer Berlin Heidelberg,Springer,Springer Nature B.V
• Subject: Alcohol / Alcohols / analogs & derivatives / Applied Microbial and Cell Physiology / Biomedical and Life Sciences / biosurfactants / Biotechnology / Carbon / Carbon sources / Chemical properties / chemistry / Chromatography / classification / diastereomers / DNA, Fungal / DNA, Fungal - chemistry / DNA, Fungal - genetics / DNA, Ribosomal Spacer / DNA, Ribosomal Spacer - chemistry / DNA, Ribosomal Spacer - genetics / Erythritol / Erythritol - analogs & derivatives / Erythritol - metabolism / foods / genetics / Glucose / Glycerol / Identification and classification / industry / Life Sciences / Lipids / Mannosides / Mannosides - metabolism / metabolism / Microbial Genetics and Genomics / Microbiological chemistry / Microbiological research / Microbiology / Microorganisms / Molecular Sequence Data / Morphology / NMR / Nuclear magnetic resonance / Physiological aspects / Physiology / Pseudozyma / Sequence Analysis, DNA / Stereoisomerism / Studies / Sugar / Sugar Alcohols / Sugar Alcohols - metabolism / Sugars / Surfactants / Ustilaginales / Ustilaginales - classification / Ustilaginales - genetics / Ustilaginales - metabolism / Vegetable oils / Yeast / Yeast fungi / Yeasts