Journal Article

Assessment of Domestic Goats as Models for Experimental and Natural Infection with the North American Isolate of Rickettsia slovaca

Powell, Nathaniel, Keating, M. Kelly, Lathrop, George W., Lukovsky-Akhsanov, Nicole, Spivey, Pamela, Levin, Michael L.

2016

Overview

Rickettsia slovaca is a tick-borne human pathogen that is associated with scalp eschars and neck lymphadenopathy known as tick-borne lymphadenopathy (TIBOLA) or Dermacentor-borne necrosis erythema and lymphadenopathy (DEBONEL). Originally, R. slovaca was described in Eastern Europe, but since recognition of its pathogenicity, human cases have been reported throughout Europe. European vertebrate reservoirs of R. slovaca remain unknown, but feral swine and domestic goats have been found infected or seropositive for this pathogen. Recently, a rickettsial pathogen identical to R. slovaca was identified in, and isolated from, the American dog tick, Dermacentor variabilis. In previous experimental studies, this organism was found infectious to guinea pigs and transovarially transmissible in ticks. In this study, domestic goats (Capra hircus) were experimentally inoculated with the North American isolate of this R. slovaca-like agent to assess their reservoir competence-the ability to acquire the pathogens and maintain transmission between infected and uninfected ticks. Goats were susceptible to infection as demonstrated by detection of the pathogen in skin biopsies and multiple internal tissues, but the only clinical sign of illness was transient fever noted in three out of four goats, and reactive lymphoid hyperplasia. On average, less than 5% of uninfected ticks acquired the pathogen while feeding upon infected goats. Although domestic goats are susceptible to the newly described North American isolate of R. slovaca, they are likely to play a minor role in the natural transmission cycle of this pathogen. Our results suggest that goats do not propagate the North American isolate of R. slovaca in peridomestic environments and clinical diagnosis of infection could be difficult due to the brevity and mildness of clinical signs. Further research is needed to elucidate the natural transmission cycle of R. slovaca both in Europe and North America, as well as to identify a more suitable laboratory model.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Animal models / Animals / Animals, Domestic / Arachnids / Biology and Life Sciences / Body Temperature / Capra hircus / Cell culture / Dermacentor marginatus / Dermacentor reticulatus / Dermacentor variabilis / Disease control / Disease Models, Animal / Disease prevention / Disease susceptibility / Disease transmission / DNA, Bacterial - isolation & purification / DNA, Bacterial - metabolism / Domestic animals / Erythema / Feeds / Female / Feral populations / Fever / Goats / Guinea pigs / Hyperplasia / Infections / Infectious diseases / Ixodidae / Laboratory animals / Livestock / Lymphadenopathy / Lymphatic system / Male / Medicine and Health Sciences / Neck / North America / Parasitic diseases / Pathogenicity / Pathogens / Pathology / Polymerase Chain Reaction / Research and Analysis Methods / Rickettsia / Rickettsia - genetics / Rickettsia - isolation & purification / Rickettsia - pathogenicity / Rickettsia Infections - microbiology / Rickettsia Infections - pathology / Rickettsia Infections - transmission / Rickettsia raoultii / Rickettsia slovaca / Scalp / Skin / Skin - microbiology / Skin - pathology / Ticks / Ticks - microbiology / Tissues / Vectors (Biology) / Zoonoses