Journal Article

Downregulation of HOTAIR Expression Mediated Anti-Metastatic Effect of Artesunate on Cervical Cancer by Inhibiting COX-2 Expression

Qian, Hua, Lin, Mei, Yuan, Donglan, Sha, Min, Luan, Zhengyun, Ye, Lihua, Huang, Junxing, Li, Xiaokang, Zhang, Lixin

2016

Overview

Artesunate (ART) has anti-cancer activities for a variety of solid tumors. The aim of this study was to investigate the anti-metastatic effect of ART on cervical cancer cells. In vivo anti-metastatic effect of ART was investigated in mice with the lung metastasis model by the subcutaneous injection of ART. The interaction of HOTAIR and COX-2 was measured by RNA immunoprecipitation and RNA pull-down assay. The effect of ART on metastasis of CaSki and Hela cells was evaluated by invasion and migration assay. We found that ART inhibited cervical cancer metastasis and HOTAIR expression. HOTAIR overexpression partially abolished the anti-metastatic effect of ART on cervical cancer cells. In addition, HOTAIR can interact with COX-2 to positively regulate COX-2 expression and catalytic activity. Finally, overexpression of COX-2 reversed the effect of HOTAIR knockdown on Hela cell migration and invasion. Taken together, our data revealed that ART may elicit anti-metastatic effect against cervical cancer by inhibition of HOTAIR expression, which resulted in the decrease of COX-2 expression.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Angiogenesis / Animals / Anticancer properties / Antineoplastic Agents - chemistry / Antineoplastic Agents - therapeutic use / Antineoplastic Agents - toxicity / Artemisinins - chemistry / Artemisinins - therapeutic use / Artemisinins - toxicity / Artesunate / Biology and Life Sciences / Cancer / Cancer metastasis / Cancer prevention / Cancer therapies / Cancer treatment / Catalysis / Catalytic activity / Cell cycle / Cell Line, Tumor / Cell migration / Cell Movement - drug effects / Cervical cancer / Cervix / Clinical medicine / Cyclooxygenase 2 - chemistry / Cyclooxygenase 2 - metabolism / Cyclooxygenase-2 / Dinoprostone - metabolism / Down-Regulation - drug effects / Female / Gene Expression Regulation, Neoplastic - drug effects / Gynecology / HeLa Cells / Human papillomavirus / Humans / Immunoprecipitation / In vivo methods and tests / Laboratory animals / Lung Neoplasms - prevention & control / Lung Neoplasms - secondary / Lungs / Lymphatic system / Medical prognosis / Medicine / Medicine and Health Sciences / Metastases / Metastasis / Mice / Mice, Nude / Protein Binding / Proteins / Reproductive health / Research and analysis methods / Ribonucleic acid / RNA / RNA Interference / RNA, Long Noncoding - antagonists & inhibitors / RNA, Long Noncoding - genetics / RNA, Long Noncoding - metabolism / RNA, Small Interfering - metabolism / Solid tumors / Transplantation, Heterologous / Tumors / Uterine Cervical Neoplasms - drug therapy / Uterine Cervical Neoplasms - metabolism / Uterine Cervical Neoplasms - pathology / Vascular endothelial growth factor