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The single-cell transcriptional landscape of mammalian organogenesis
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The single-cell transcriptional landscape of mammalian organogenesis
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Journal Article
The single-cell transcriptional landscape of mammalian organogenesis
2019
Overview
Mammalian organogenesis is a remarkable process. Within a short timeframe, the cells of the three germ layers transform into an embryo that includes most of the major internal and external organs. Here we investigate the transcriptional dynamics of mouse organogenesis at single-cell resolution. Using single-cell combinatorial indexing, we profiled the transcriptomes of around 2 million cells derived from 61 embryos staged between 9.5 and 13.5 days of gestation, in a single experiment. The resulting ‘mouse organogenesis cell atlas’ (MOCA) provides a global view of developmental processes during this critical window. We use Monocle 3 to identify hundreds of cell types and 56 trajectories, many of which are detected only because of the depth of cellular coverage, and collectively define thousands of corresponding marker genes. We explore the dynamics of gene expression within cell types and trajectories over time, including focused analyses of the apical ectodermal ridge, limb mesenchyme and skeletal muscle.
Data from single-cell combinatorial-indexing RNA-sequencing analysis of 2 million cells from mouse embryos between embryonic days 9.5 and 13.5 are compiled in a cell atlas of mouse organogenesis, which provides a global view of developmental processes occurring during this critical period.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 38/91
/ 64
/ 64/60
/ Animals
/ Embryo
/ Embryo, Mammalian - cytology
/ Embryo, Mammalian - embryology
/ Embryo, Mammalian - metabolism
/ Embryos
/ Female
/ Gene Expression Regulation, Developmental - genetics
/ Genes
/ Humanities and Social Sciences
/ Male
/ Mammals
/ Mice
/ Muscle Development - genetics
/ Muscle, Skeletal - embryology
/ Muscle, Skeletal - metabolism
/ Muscles
/ Organ Specificity - genetics
/ Organs
/ RNA
/ Science
