Journal Article

Excretory/Secretory Products from Trichinella spiralis Adult Worms Ameliorate DSS-Induced Colitis in Mice

Cheng, Yuli, Yang, Xiaodi, Gu, Yuan, Yang, Yaping, Wang, Yunyun, Zhu, Xinping, Zhan, Bin

2014

Overview

Many evidences show the inverse correlation between helminth infection and allergic or autoimmune diseases. Identification and characterization of the active helminth-derived products responsible for the beneficial effects on allergic or inflammatory diseases will provide another feasible approach to treat these diseases. Colitis was induced in C57BL/6 mice by giving 3% DSS orally for 7 days. During this period, the mice were treated daily with the excretory/secretory products from T. spiralis adult worms (AES) intraperitoneally. The severity of colitis was monitored by measuring body weight, stool consistency or bleeding, colon length and inflammation. To determine the T. spiralis AES product-induced immunological response, Th1, Th2, Th17 and regulatory cytokine profiles were measured in lymphocytes isolated from colon, mesenteric lymph nodes (MLN), and the spleen of treated mice. The CD4+ CD25+ FOXP3+ regulatory T cells (Tregs) were also measured in the spleens and MLN of treated mice. Mice treated with AES significantly ameliorated the severity of the DSS-induced colitis indicated by the reduced disease manifestations, improved macroscopic and microscopic inflammation correlated with the up-regulation of Treg response (increased regulatory cytokines IL-10, TGF-beta and regulatory T cells) and down-regulation of pro-inflammatory cytokines (IFN-gamma, IL-6 and IL-17) in the spleens, MLN and colon of treated mice. Our results provide direct evidences that T. spiralis AES have a therapeutic potential for alleviating inflammatory colitis in mice. This effect is possibly mediated by the immunomodulation of regulatory T cells to produce regulatory and anti-inflammatory cytokines and inhibit pro-inflammatory cytokines.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animals / Antigens / Autoimmune diseases / Biological Products - administration & dosage / Biological Products - pharmacology / Biology and Life Sciences / Bleeding / Body weight / Body Weight - drug effects / Bone morphogenetic proteins / CD25 antigen / CD4 antigen / Colitis / Colitis - chemically induced / Colitis - prevention & control / Colon / Colon - drug effects / Colon - metabolism / Colon - pathology / Cytokines / Cytokines - metabolism / Dendritic cells / Dextran Sulfate / Diarrhea - prevention & control / Drinking water / Female / Flow Cytometry / Forkhead Transcription Factors - metabolism / Foxp3 protein / Health aspects / Helper cells / House mouse / Hypotheses / Immune system / Immunology / Immunomodulation / Immunoregulation / Infections / Inflammation / Inflammatory bowel disease / Inflammatory diseases / Injections, Intraperitoneal / Interferon / Interleukin 10 / Interleukin 17 / Interleukin 6 / Interleukin-2 Receptor alpha Subunit - metabolism / Laboratory animals / Lymph nodes / Lymph Nodes - drug effects / Lymph Nodes - metabolism / Lymphocytes / Lymphocytes - drug effects / Lymphocytes - metabolism / Lymphocytes T / Medicine and Health Sciences / Mice / Mice, Inbred C57BL / Necator americanus / Parasites / Parasitology / Proteins / Research and Analysis Methods / Rodents / Schistosoma mansoni / Spleen / Spleen - drug effects / Spleen - metabolism / Studies / T cells / T-Lymphocytes, Helper-Inducer - drug effects / T-Lymphocytes, Helper-Inducer - metabolism / T-Lymphocytes, Regulatory - drug effects / T-Lymphocytes, Regulatory - metabolism / Transforming growth factor-b / Transforming growth factors / Treatment Outcome / Trichinella spiralis / Trichinella spiralis - chemistry / Worms