Asset Details
Do you wish to reserve the book?
Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins
Do you wish to request the book?
Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins
2013
Overview
Acinetobacter baumannii is increasingly becoming a major nosocomial pathogen. This opportunistic pathogen secretes outer membrane vesicles (OMVs) that interact with host cells. The aim of this study was to investigate the ability of A. baumannii OMVs to elicit a pro-inflammatory response in vitro and the immunopathology in response to A. baumannii OMVs in vivo. OMVs derived from A. baumannii ATCC 19606(T) induced expression of pro-inflammatory cytokine genes, interleukin (IL)-1β and IL-6, and chemokine genes, IL-8, macrophage inflammatory protein-1α, and monocyte chemoattractant protein-1, in epithelial cells in a dose-dependent manner. Disintegration of OMV membrane with ethylenediaminetetraacetic acid resulted in low expression of pro-inflammatory cytokine genes, as compared with the response to intact OMVs. In addition, proteinase K-treated A. baumannii OMVs did not induce significant increase in expression of pro-inflammatory cytokine genes above the basal level, suggesting that the surface-exposed membrane proteins in intact OMVs are responsible for pro-inflammatory response. Early inflammatory processes, such as vacuolization and detachment of epithelial cells and neutrophilic infiltration, were clearly observed in lungs of mice injected with A. baumannii OMVs. Our data demonstrate that OMVs produced by A. baumannii elicit a potent innate immune response, which may contribute to immunopathology of the infected host.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Acinetobacter baumannii - drug effects
/ Acinetobacter baumannii - immunology
/ Animals
/ Bacteria
/ Bacterial Outer Membrane Proteins - immunology
/ Biofilms
/ Biology
/ Cell Membrane - drug effects
/ Endopeptidase K - pharmacology
/ Epithelial Cells - drug effects
/ Epithelial Cells - immunology
/ Epithelial Cells - microbiology
/ Epithelial Cells - pathology
/ Female
/ Genes
/ Humans
/ Immunity, Innate - drug effects
/ Immunity, Innate - immunology
/ Lungs
/ Macrophage inflammatory protein
/ Medicine
/ Mice
/ Monocyte chemoattractant protein
/ Monocyte chemoattractant protein 1
/ Proteins
/ Secretory Vesicles - drug effects
/ Secretory Vesicles - immunology
/ Vesicles
