Journal Article

Influence of inflammasome NLRP3, and IL1B and IL2 gene polymorphisms in periodontitis susceptibility

de Alencar, Josiane Bazzo, Souza, Victor Hugo de, Visentainer, Jeane Eliete Laguila, Silva, Cléverson de Oliveira e, Sell, Ana Maria, Zacarias, Joana Maira Valentini, Tsuneto, Patrícia Yumeko

2020

Overview

The pathogenesis of periodontitis (PD) involves several molecules of the immune system that interact in a network to eliminate the periodontopathogens, yet, they contribute to periodontal tissue destruction. The different mechanisms that lead to periodontal tissue damage are not clear. Despite this, immune response genes have been related to the development of PD previously, such as those involved in inflammasomes which are multiprotein complexes and cytokines including Interleukin-1. The aim of the study was to evaluate the polymorphisms in NLRP3 inflammasome, cytokine and receptor of cytokines genes in the development of periodontitis. This case-control study was conducted in 186 patients with PD (stage II and III and grade B) and 208 controls (localized gingivitis and periodontally healthy individuals). Genotyping was performed using PCR-RFLP for the SNP rs4612666 in NLRP3 and using PCR-SSP for IL1A, IL1B, IL1R, IL1RN, IL4RA, INFG, TGFB1, TNF, IL2, IL4, IL6, and IL10. Cytokine serum levels were measured using Luminex technology. SNPStats and OpenEpi software were used to perform statistical analysis. The higher frequencies of NLRP3 T/C and IL1B -511 T/T genotypes and IL2 (+166, -330) GT haplotype were observed in patients with PD compared to controls. The SNPs in NLRP3, IL1R +1970, IL6-174, TNF -308, IL2 +166 and -330, TGFB1 +869 and +915, IL4RA +1902, IL4-1098 and -590 were associated to PD in men. In conclusion, polymorphisms in NLRP3, IL1B and IL2 genes were associated to PD susceptibility. Men carrying the NLRP3, IL1R, IL6, TNF, IL2, TGFB1, IL4RA and IL4 polymorphisms had greater susceptibility than women for developing PD.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adult / Analysis / B cells / Biology and Life Sciences / Cytokines / Cytokines - genetics / Dentistry / Diabetes / Disease susceptibility / EDTA / Female / Fibroblasts / Genes / Genetic aspects / Genetic Association Studies / Genetic polymorphisms / Genetic Predisposition to Disease / Genotype / Genotypes / Genotyping / Gingivitis / Gum disease / Haplotypes / Haplotypes - genetics / Health sciences / Humans / Immune response / Immune system / Inflammasomes / Inflammasomes - genetics / Interleukin / Interleukin 1 / Interleukin 1 receptors / Interleukin 10 / Interleukin 2 / Interleukin 4 / Interleukin 6 / Interleukin-1beta - genetics / Interleukin-2 - genetics / Interleukins / Male / Medical research / Medicine and Health Sciences / Middle Aged / NLR Family, Pyrin Domain-Containing 3 Protein - genetics / Pathogenesis / People and places / Periodontitis / Periodontitis - genetics / Periodontitis - pathology / Periodontium / Polymerase chain reaction / Polymorphism, Restriction Fragment Length - genetics / Polymorphism, Single Nucleotide - genetics / Population / Restriction fragment length polymorphism / Rheumatic diseases / Serum levels / Sex Characteristics / Single-nucleotide polymorphism / Social Sciences / Statistical analysis / Technology / Transforming Growth Factor beta1 - genetics / Transforming growth factor-b1 / Tumor necrosis factor / Tumor necrosis factor-TNF