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Identification of novel GAPDH-derived antimicrobial peptides secreted by saccharomyces cerevisiae and involved in wine microbial interactions
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Identification of novel GAPDH-derived antimicrobial peptides secreted by saccharomyces cerevisiae and involved in wine microbial interactions
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Journal Article
Identification of novel GAPDH-derived antimicrobial peptides secreted by saccharomyces cerevisiae and involved in wine microbial interactions
2014
Overview
Saccharomyces cerevisiae plays a primordial role in alcoholic fermentation and has a vast worldwide application in the production of fuel-ethanol, food and beverages. The dominance of S. cerevisiae over other microbial species during alcoholic fermentations has been traditionally ascribed to its higher ethanol tolerance. However, recent studies suggested that other phenomena, such as microbial interactions mediated by killer-like toxins, might play an important role. Here we show that S. cerevisiae secretes antimicrobial peptides (AMPs) during alcoholic fermentation that are active against a wide variety of wine-related yeasts (e.g. Dekkera bruxellensis) and bacteria (e.g. Oenococcus oeni). Mass spectrometry analyses revealed that these AMPs correspond to fragments of the S. cerevisiae glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein. The involvement of GAPDH-derived peptides in wine microbial interactions was further sustained by results obtained in mixed cultures performed with S. cerevisiae single mutants deleted in each of the GAPDH codifying genes (TDH1-3) and also with a S. cerevisiae mutant deleted in the YCA1 gene, which codifies the apoptosis-involved enzyme metacaspase. These findings are discussed in the context of wine microbial interactions, biopreservation potential and the role of GAPDH in the defence system of S. cerevisiae.
Publisher
Springer Verlag,HAL CCSD,Springer-Verlag,Springer Berlin Heidelberg,Springer,Springer Nature B.V
Subject
/ Antimicrobial Cationic Peptides
/ Antimicrobial Cationic Peptides - secretion
/ Applied Microbial and Cell Physiology
/ Bacteria
/ Biomedical and Life Sciences
/ Dekkera
/ Ethanol
/ genes
/ glyceraldehyde-3-phosphate dehydrogenase
/ Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
/ Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - secretion
/ Microbial Genetics and Genomics
/ mutants
/ Peptides
/ Saccharomyces cerevisiae - growth & development
/ Saccharomyces cerevisiae - metabolism
/ Toxins
/ Vitaceae
/ Wine
/ Wines
/ Yeast
/ yeasts
ISBN
0003296276000
