Journal Article

Pathogenesis and transmission of avian influenza A (H7N9) virus in ferrets and mice

Pearce, Melissa B., Belser, Jessica A., Carney, Paul J., Maines, Taronna R., Sun, Xiangjie, Zeng, Hui, Pappas, Claudia, Stevens, James, Katz, Jacqueline M., Villanueva, Julie M., Gustin, Kortney M., Tumpey, Terrence M.

2013

Overview

The new H7N9 influenza virus, recently emerged in China, can replicate in human airway cells and in the respiratory tract of ferrets to a higher level than can seasonal H3N2 virus and shows higher lethality in mice than genetically related H7N9 and H9N2 viruses, but shows limited transmission in ferrets by respiratory droplets. Transmission of emerging H7N9 virus By 20 July 2013, there had been 134 laboratory-confirmed human cases of infection with avian influenza A H7N9 virus infection, including 43 deaths. Yoshihiro Kawaoka and colleagues characterize the biology of two recent isolates of the virus. They provide a wealth of data from infections in mice, pigs, macaques and ferrets. H7N9 virus is shown to be less sensitive to neuraminidase inhibitors than pandemic H1N1 virus, but equally susceptible to an experimental polymerase inhibitor. Terrence Tumpey and colleagues determine the capacity of two clinical H7N9 isolates to cause disease and transmit between mammals. They show that the virus can replicate in human airway cells and in the respiratory tract of ferrets to a higher level than can seasonal H3N2 virus, and show higher lethality in mice than genetically related H7N9 and H9N2 viruses. In transmission studies, the H7N9 virus showed limited transmission in ferrets by respiratory droplets. Ron Fouchier and colleagues investigate the transmissibility of H7N9 virus between ferrets. They show that airborne transmission can occur, but inefficiently. They also show that on passage in ferrets, virus variants that have higher avian receptor binding, higher pH of fusion and lower thermostability are selected, and they suggest that these characteristics may result in reduced transmissibility. On 29 March 2013, the Chinese Center for Disease Control and Prevention confirmed the first reported case of human infection with an avian influenza A(H7N9) virus 1 . The recent human infections with H7N9 virus, totalling over 130 cases with 39 fatalities to date, have been characterized by severe pulmonary disease and acute respiratory distress syndrome (ARDS) 2 . This is concerning because H7 viruses have typically been associated with ocular disease in humans, rather than severe respiratory disease 3 . This recent outbreak underscores the need to better understand the pathogenesis and transmission of these viruses in mammals. Here we assess the ability of A/Anhui/1/2013 and A/Shanghai/1/2013 (H7N9) viruses, isolated from fatal human cases, to cause disease in mice and ferrets and to transmit to naive animals. Both H7N9 viruses replicated to higher titre in human airway epithelial cells and in the respiratory tract of ferrets compared to a seasonal H3N2 virus. Moreover, the H7N9 viruses showed greater infectivity and lethality in mice compared to genetically related H7N9 and H9N2 viruses. The H7N9 viruses were readily transmitted to naive ferrets through direct contact but, unlike the seasonal H3N2 virus, did not transmit readily by respiratory droplets. The lack of efficient respiratory droplet transmission was corroborated by low receptor-binding specificity for human-like α2,6-linked sialosides. Our results indicate that H7N9 viruses have the capacity for efficient replication in mammals and human airway cells and highlight the need for continued public health surveillance of this emerging virus.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 631/326/596/2563 / Access control / Animals / Avian influenza / Avian influenza viruses / Causes of / Cell Line / Cell Polarity / Demographic aspects / Disease Models, Animal / Disease transmission / Diseases / Epidemics / Epithelial Cells - virology / Eye diseases / Fatalities / Female / Ferrets - virology / Health aspects / Humanities and Social Sciences / Humans / Illnesses / Infections / Influenza / Influenza A virus - growth & development / Influenza A virus - isolation & purification / Influenza A virus - metabolism / Influenza A virus - pathogenicity / Influenza A Virus, H3N2 Subtype - growth & development / Influenza A Virus, H3N2 Subtype - pathogenicity / Influenza A Virus, H9N2 Subtype - growth & development / Influenza A Virus, H9N2 Subtype - pathogenicity / Influenza viruses / Influenza, Human - virology / Laboratories / letter / Madin Darby Canine Kidney Cells / Male / Mammals / Mice - virology / Mice, Inbred BALB C / multidisciplinary / Orthomyxoviridae Infections - transmission / Orthomyxoviridae Infections - virology / Pathogenesis / Polysaccharides - chemistry / Polysaccharides - metabolism / Public health / Public health movements / Receptors, Virus - chemistry / Receptors, Virus - metabolism / Respiratory diseases / Respiratory distress syndrome / Respiratory System - cytology / Respiratory tract / Science / Substrate Specificity / Swine / Virus diseases / Virus Replication - physiology / Viruses