Journal Article

A complement–microglial axis drives synapse loss during virus-induced memory impairment

Robinson, John K., Garber, Charise, Garbow, Joel R., Soung, Allison, Suthar, Mehul S., Dorsey, Denise, Vasek, Michael J., Durrant, Douglas M., Wilton, Daniel K., Perez-Torres, Carlos, Frouin, Arnaud, Bollman, Bryan, Funk, Kristen, Stevens, Beth, Mennerick, Steven, Klein, Robyn S., Jiang, Xiaoping, Bowen, James R., Schmidt, Robert E., Tyler, Kenneth L., Yu, Jinsheng, DeMasters, Bette K.

2016

Overview

People infected with West Nile virus often experience cognitive side effects including memory loss through unknown mechanisms; mice and humans infected with the virus experience a loss in hippocampal presynaptic terminals, which can be reversed by disrupting complement or microglia in mice. Cognitive abnormalities associated with West Nile virus A majority of West Nile virus (WNV) sufferers experience cognitive signs and symptoms, including memory dysfunction, but the mechanisms driving these impairments are largely unknown. Robyn Klein and colleagues demonstrate an enhancement of complement-mediated synaptic pruning in the hippocampus following WNV infection. This pruning required microglia and resembled developmental pruning by the same mechanism. Disruption of complement or microglia during infection protected animals from the WNV-induced memory deficits. Over 50% of patients who survive neuroinvasive infection with West Nile virus (WNV) exhibit chronic cognitive sequelae 1 , 2 . Although thousands of cases of WNV-mediated memory dysfunction accrue annually 3 , the mechanisms responsible for these impairments are unknown. The classical complement cascade, a key component of innate immune pathogen defence, mediates synaptic pruning by microglia during early postnatal development 4 , 5 . Here we show that viral infection of adult hippocampal neurons induces complement-mediated elimination of presynaptic terminals in a murine WNV neuroinvasive disease model. Inoculation of WNV-NS5-E218A, a WNV with a mutant NS5(E218A) protein 6 , 7 leads to survival rates and cognitive dysfunction that mirror human WNV neuroinvasive disease. WNV-NS5-E218A-recovered mice (recovery defined as survival after acute infection) display impaired spatial learning and persistence of phagocytic microglia without loss of hippocampal neurons or volume. Hippocampi from WNV-NS5-E218A-recovered mice with poor spatial learning show increased expression of genes that drive synaptic remodelling by microglia via complement. C1QA was upregulated and localized to microglia, infected neurons and presynaptic terminals during WNV neuroinvasive disease. Murine and human WNV neuroinvasive disease post-mortem samples exhibit loss of hippocampal CA3 presynaptic terminals, and murine studies revealed microglial engulfment of presynaptic terminals during acute infection and after recovery. Mice with fewer microglia ( Il34 −/− mice with a deficiency in IL-34 production) or deficiency in complement C3 or C3a receptor were protected from WNV-induced synaptic terminal loss. Our study provides a new murine model of WNV-induced spatial memory impairment, and identifies a potential mechanism underlying neurocognitive impairment in patients recovering from WNV neuroinvasive disease.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 13 / 14 / 14/19 / 14/28 / 38 / 38/1 / 38/32 / 38/39 / 38/61 / 38/77 / 45 / 59 / 59/57 / 631/250/371 / 631/326/596/2553 / 631/378/1595/1554 / 631/378/340 / 64 / 64/60 / 82 / 82/51 / Analysis / Animals / Apoptosis / CA3 Region, Hippocampal - immunology / CA3 Region, Hippocampal - pathology / CA3 Region, Hippocampal - virology / Cognition disorders / Complement Activation / Complement Pathway, Classical - immunology / Complement System Proteins - immunology / Complications and side effects / Diagnosis / Disease Models, Animal / Female / Health aspects / Humanities and Social Sciences / Humans / letter / Male / Medical research / Medicine, Experimental / Memory / Memory Disorders - immunology / Memory Disorders - pathology / Memory Disorders - physiopathology / Memory Disorders - virology / Mice / Microglia - immunology / multidisciplinary / Neuronal Plasticity / Neurons / Neurons - immunology / Neurons - pathology / Neurons - virology / Neurophysiology / Presynaptic Terminals - immunology / Presynaptic Terminals - pathology / Proteins / Rodents / Science / Spatial Memory / Studies / Survival / Vector-borne diseases / Viral infections / West Nile fever / West Nile Fever - pathology / West Nile Fever - physiopathology / West Nile Fever - virology / West Nile virus / West Nile virus - immunology / West Nile virus - pathogenicity