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A carboxylesterase, Esterase-6, modulates sensory physiological and behavioral response dynamics to pheromone in Drosophila
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A carboxylesterase, Esterase-6, modulates sensory physiological and behavioral response dynamics to pheromone in Drosophila
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A carboxylesterase, Esterase-6, modulates sensory physiological and behavioral response dynamics to pheromone in Drosophila
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Journal Article
A carboxylesterase, Esterase-6, modulates sensory physiological and behavioral response dynamics to pheromone in Drosophila
2012
Overview
Background
Insects respond to the spatial and temporal dynamics of a pheromone plume, which implies not only a strong response to 'odor on', but also to 'odor off'. This requires mechanisms geared toward a fast signal termination. Several mechanisms may contribute to signal termination, among which odorant-degrading enzymes. These enzymes putatively play a role in signal dynamics by a rapid inactivation of odorants in the vicinity of the sensory receptors, although direct
in vivo
experimental evidences are lacking. Here we verified the role of an extracellular carboxylesterase, esterase-6 (Est-6), in the sensory physiological and behavioral dynamics of
Drosophila melanogaster
response to its pheromone,
cis
-vaccenyl acetate (cVA). Est-6 was previously linked to post-mating effects in the reproductive system of females. As Est-6 is also known to hydrolyze cVA
in vitro
and is expressed in the main olfactory organ, the antenna, we tested here its role in olfaction as a putative odorant-degrading enzyme.
Results
We first confirm that
Est-6
is highly expressed in olfactory sensilla, including cVA-sensitive sensilla, and we show that expression is likely associated with non-neuronal cells. Our electrophysiological approaches show that the dynamics of olfactory receptor neuron (ORN) responses is strongly influenced by Est-6, as in
Est-6°
null mutants (lacking the
Est-6
gene) cVA-sensitive ORN showed increased firing rate and prolonged activity in response to cVA.
Est-6°
mutant males had a lower threshold of behavioral response to cVA, as revealed by the analysis of two cVA-induced behaviors. In particular, mutant males exhibited a strong decrease of male-male courtship, in association with a delay in courtship initiation.
Conclusions
Our study presents evidence that
Est-6
plays a role in the physiological and behavioral dynamics of sex pheromone response in
Drosophila
males and supports a role of
Est-6
as an odorant-degrading enzyme (ODE) in male antennae. Our results also expand the role of
Est-6
in
Drosophila
biology, from reproduction to olfaction, and highlight the role of ODEs in insect olfaction.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Analysis
/ Animals
/ Antennae
/ Arthropod Antennae - enzymology
/ Behavior
/ Behavior, Animal - drug effects
/ Biomedical and Life Sciences
/ Carboxylesterase - metabolism
/ Drosophila melanogaster - drug effects
/ Drosophila melanogaster - enzymology
/ Drosophila melanogaster - physiology
/ Drosophila Proteins - metabolism
/ Enzims
/ Enzymes
/ Etologia
/ Female
/ Females
/ Food
/ Male
/ Males
/ Odorants
/ Odors
/ Olfactory Receptor Neurons - drug effects
/ Olfactory Receptor Neurons - physiology
/ Olors
/ Plumes
/ Sensilla
