Asset Details
Do you wish to reserve the book?
Extracellular matrix formation enhances the ability of Streptococcus pneumoniae to cause invasive disease
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Extracellular matrix formation enhances the ability of Streptococcus pneumoniae to cause invasive disease
Do you wish to request the book?
Extracellular matrix formation enhances the ability of Streptococcus pneumoniae to cause invasive disease
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Extracellular matrix formation enhances the ability of Streptococcus pneumoniae to cause invasive disease
2011
Overview
During infection, pneumococci exist mainly in sessile biofilms rather than in planktonic form, except during sepsis. However, relatively little is known about how biofilms contribute to pneumococcal pathogenesis. Here, we carried out a biofilm assay on opaque and transparent variants of a clinical serotype 19F strain WCH159. After 4 days incubation, scanning electron microscopy revealed that opaque biofilm bacteria produced an extracellular matrix, whereas the transparent variant did not. The opaque biofilm-derived bacteria translocated from the nasopharynx to the lungs and brain of mice, and showed 100-fold greater in vitro adherence to A549 cells than transparent bacteria. Microarray analysis of planktonic and sessile bacteria from transparent and opaque variants showed differential gene expression in two operons: the lic operon, which is involved in choline uptake, and in the two-component system, ciaRH. Mutants of these genes did not form an extracellular matrix, could not translocate from the nasopharynx to the lungs or the brain, and adhered poorly to A549 cells. We conclude that only the opaque phenotype is able to form extracellular matrix, and that the lic operon and ciaRH contribute to this process. We propose that during infection, extracellular matrix formation enhances the ability of pneumococci to cause invasive disease.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Analysis
/ Animals
/ Antigens
/ Bacteria
/ Bacterial Capsules - metabolism
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biofilms
/ Biofilms - growth & development
/ Biology
/ Brain
/ Choline
/ Enzyme-Linked Immunosorbent Assay
/ Extracellular Matrix - metabolism
/ Extracellular Matrix - ultrastructure
/ Gene Expression Regulation, Bacterial
/ Humans
/ Lungs
/ Methods
/ Mice
/ Mutants
/ Mutation
/ Operons
/ Pneumococcal Infections - microbiology
/ Scanning electron microscopy
/ Sepsis
/ Streptococcus pneumoniae - genetics
/ Streptococcus pneumoniae - growth & development
/ Streptococcus pneumoniae - physiology
/ Streptococcus pneumoniae - ultrastructure
/ Vaccines
