Journal Article

effect of fungicides on yeast communities associated with grape berries

Čadež, Neža, Raspor, Peter, Zupan, Jure

2010

Overview

The influence of three commonly used fungicides (iprodione, pyrimethanil and fludioxonil plus cyprodinil) on the density and diversity of yeast populations present on grape berries was evaluated. At the time of harvest, the fungicide residues on grapes were below the maximum permitted levels. In general, larger yeast counts were found on the treated grapes than on the control samples. Among 23 species identified, Cryptococcus magnus, Rhodotorula glutinis and Sporidiobolus pararoseus dominated on sound grape berries. The results showed that the tested fungicides had only a minor impact on the composition of grape berry communities in comparison with the effect of weather conditions and the mode of grape berry sampling. Halo assays using filter discs loaded with fungicides were used as in vitro tests of the sensitivity of grape berry isolates. The fungicide containing pyrimethanil suppressed the growth of all basidiomycetous yeast species, while the sporadically occurring fermentative yeasts were unaffected. Fungicides with fludioxonil plus cyprodinil and iprodione as active substances showed specificity for certain species. Our results suggest that after the safety interval, the presence of fungicides has a minor impact on the composition of grape berry communities, although at the time of fungicide applications, the yeast species composition changes.

• Publisher: Oxford, UK : Blackwell Publishing Ltd,Blackwell Publishing Ltd,Oxford University Press
• Subject: active ingredients / basidiomycetes / Basidiomycota / Berries / Biodiversity / chemistry / classification / Cryptococcus laurentii magnus / Cryptococcus magnus / cyprodinil / DNA, Fungal / DNA, Fungal - chemistry / DNA, Fungal - genetics / DNA, Ribosomal / DNA, Ribosomal - chemistry / DNA, Ribosomal - genetics / DNA, Ribosomal Spacer / DNA, Ribosomal Spacer - chemistry / DNA, Ribosomal Spacer - genetics / drug effects / Fludioxonil / Fruits / Fungi / Fungi - classification / Fungi - drug effects / Fungi - isolation & purification / fungicide / fungicide residues / Fungicides / Fungicides, Industrial / Fungicides, Industrial - pharmacology / Genes, rRNA / genetics / grape berry / grapes / halo assay / harvest date / in vitro studies / Iprodione / isolation & purification / Microbial Sensitivity Tests / microbiology / Molecular Sequence Data / pesticide application / Pesticides / pharmacology / polyphasic identification / pyrimethanil / Rhodotorula glutinis / RNA, Fungal / RNA, Fungal - genetics / RNA, Ribosomal, 5.8S / RNA, Ribosomal, 5.8S - genetics / Sequence Analysis, DNA / Species composition / species diversity / Sporidiobolus / Sporidiobolus pararoseus / Vitaceae / Vitis / Vitis - microbiology / Weather / wine yeast / wine yeasts / Yeast / yeasts