Journal Article

Optogenetic stimulation of a hippocampal engram activates fear memory recall

Puryear, Corey B., Liu, Xu, Tonegawa, Susumu, Govindarajan, Arvind, Deisseroth, Karl, Pang, Petti T., Ramirez, Steve

2012

Overview

The activation of a population of hippocampal neurons thought to encode a specific fear memory is shown to elicit freezing behaviour in mice. Neural representation of a memory Several studies have used ablation strategies to demonstrate that certain neuronal populations in the brain are needed for memory expression, but whether a particular ensemble is sufficient to elicit a behavioural outcome from a particular memory has remained unexplored. Now, Susumu Tonegawa and colleagues use optogenetics to demonstrate that a particular, targeted memory 'engram', or group of cells, that was active during fear-learning is sufficient to drive freezing behaviour in mice during subsequent reactivations. A specific memory is thought to be encoded by a sparse population of neurons 1 , 2 . These neurons can be tagged during learning for subsequent identification 3 and manipulation 4 , 5 , 6 . Moreover, their ablation or inactivation results in reduced memory expression, suggesting their necessity in mnemonic processes. However, the question of sufficiency remains: it is unclear whether it is possible to elicit the behavioural output of a specific memory by directly activating a population of neurons that was active during learning. Here we show in mice that optogenetic reactivation of hippocampal neurons activated during fear conditioning is sufficient to induce freezing behaviour. We labelled a population of hippocampal dentate gyrus neurons activated during fear learning with channelrhodopsin-2 (ChR2) 7 , 8 and later optically reactivated these neurons in a different context. The mice showed increased freezing only upon light stimulation, indicating light-induced fear memory recall. This freezing was not detected in non-fear-conditioned mice expressing ChR2 in a similar proportion of cells, nor in fear-conditioned mice with cells labelled by enhanced yellow fluorescent protein instead of ChR2. Finally, activation of cells labelled in a context not associated with fear did not evoke freezing in mice that were previously fear conditioned in a different context, suggesting that light-induced fear memory recall is context specific. Together, our findings indicate that activating a sparse but specific ensemble of hippocampal neurons that contribute to a memory engram is sufficient for the recall of that memory. Moreover, our experimental approach offers a general method of mapping cellular populations bearing memory engrams.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 631/1647/2253 / 631/378/1595/1554 / 631/378/1595/2636 / 631/378/87 / Animals / Bacterial Proteins - genetics / Bacterial Proteins - metabolism / Brain stimulation / Channelrhodopsins / Conditioning, Psychological - physiology / Conditioning, Psychological - radiation effects / Dentate Gyrus - cytology / Dentate Gyrus - physiology / Dentate Gyrus - radiation effects / Engrams / Fear / Fear - physiology / Fear - radiation effects / Female / Freezing Reaction, Cataleptic - physiology / Freezing Reaction, Cataleptic - radiation effects / Gene expression / Gene Expression Regulation / Hippocampus (Brain) / Hippocampus - cytology / Hippocampus - physiology / Hippocampus - radiation effects / Humanities and Social Sciences / Labeling / letter / Light / Luminescent Proteins - genetics / Luminescent Proteins - metabolism / Male / Memory / Mental Recall - radiation effects / Methods / Mice / Mice, Transgenic / multidisciplinary / Recollection (Psychology) / Rodents / Science / Science (multidisciplinary)