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Establishment of Characteristic Chromatogram of Honeysuckle Stem Formula Granules Based on the Concept of Whole Process Quality Control
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Establishment of Characteristic Chromatogram of Honeysuckle Stem Formula Granules Based on the Concept of Whole Process Quality Control
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Journal Article
Establishment of Characteristic Chromatogram of Honeysuckle Stem Formula Granules Based on the Concept of Whole Process Quality Control
2023
Overview
6 mm) chromatographic column ; gradient elution with acetonitrile-0.4% phosphoric acid solution (0.2 : 99. 8 , V/V) , column temperature at 25°C ; detection wavelength at 236 nm; flow rate 1.0 mL/min; using \" Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine\" , to evaluate the similarity of the characteristic chromatogram of erirde drug, standard decoction and formula granules of honeysuckle sem. According to Technical Requirements for Quality Control and Standard Formulation of Traditional Chinese Medicine Formula Granules and the principle of quality consistency, the characteristic chromatogram of raw materials, standard decoction and mass production granules was studied in this research, which systematically and comprehensively reflected the chemical composition and transmission law of honeysuckle stem, standard decoction and granules, in order to provide an experimental basis for the formulation of quality standard of honeysuckle stem formula granules. 2 Materials 2.1 Instruments Agilent 1260 High Performance Liquid Chromatograph (Agilent) ; XPE205/XSE-204 Electronic Analytical Balance (Mettler Toledo); KQ-500DE Numerical Control Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co. , Ltd.). 2 g-adding 25 mL of 50% ethanol solution-ultrasonic treatment (power 250 W, frequency 40 kHz) for 30 min - cooling- making up for the lost weight with 50% ethanol solution - shaking well- filtering, and taking the filtrate. 3.3 Methodological investigation 3.3.1 Precision test.
Publisher
Wu Chu (USA-China) Science and Culture Media Corporation
Subject
