Asset Details
Do you wish to reserve the book?
Role of Histone H3 Lysine 27 Methylation in X Inactivation
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Role of Histone H3 Lysine 27 Methylation in X Inactivation
Do you wish to request the book?
Role of Histone H3 Lysine 27 Methylation in X Inactivation
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Role of Histone H3 Lysine 27 Methylation in X Inactivation
2003
Overview
The Polycomb group (PcG) protein Eed is implicated in regulation of imprinted X-chromosome inactivation in extraembryonic cells but not of random X inactivation in embryonic cells. The Drosophila homolog of the Eed-Ezh2 PcG protein complex achieves gene silencing through methylation of histone H3 on lysine 27 (H3-K27), which suggests a role for H3-K27 methylation in imprinted X inactivation. Here we demonstrate that transient recruitment of the Eed-Ezh2 complex to the inactive X chromosome (Xi) occurs during initiation of X inactivation in both extraembryonic and embryonic cells and is accompanied by H3-K27 methylation. Recruitment of the complex and methylation on the Xi depend on Xist RNA but are independent of its silencing function. Together, our results suggest a role for Eed-Ezh2-mediated H3-K27 methylation during initiation of both imprinted and random X inactivation and demonstrate that H3-K27 methylation is not sufficient for silencing of the Xi.
Publisher
American Association for the Advancement of Science,The American Association for the Advancement of Science
Subject
/ Animals
/ Biological and medical sciences
/ Cells
/ Dosage Compensation, Genetic
/ Embryos
/ Female
/ Females
/ Fluorescent Antibody Technique
/ Fundamental and applied biological sciences. Psychology
/ Genes
/ Histones
/ Humans
/ In Situ Hybridization, Fluorescence
/ Lysine
/ Male
/ Mice
/ Molecular and cellular biology
/ Mutation
/ Polycomb Repressive Complex 2
/ Proteins
/ Repressor Proteins - metabolism
/ RNA
/ RNA, Untranslated - genetics
