Journal Article

Racial Disparities in the Molecular Landscape of Cancer

Heath, Elisabeth I, Ellerbrock, Angela, Separovic, Duska, Liu, Stephen V, Obeid, Elias, Lynce, Filipa, Bollig-Fischer, Aliccia, Xiu, Joanne, Vanderwalde, Ari, Reddy, Sandeep K

2018

Overview

African Americans (AA) have the highest incidence and mortality of any racial/ethnic group in the US for most cancer types. Heterogeneity in the molecular biology of cancer, as a contributing factor to this disparity, is poorly understood. To address this gap in knowledge, we explored the molecular landscape of colorectal cancer (CRC), non-small cell lung cancer (NSCLC) and high-grade glioma (HGG) from 271 AA and 636 Caucasian (CC) cases. DNA from formalin-fixed paraffin-embedded tumors was sequenced using next-generation sequencing. Additionally, we evaluated protein expression using immunohistochemistry. The Exome Aggregation Consortium Database was evaluated for known ethnicity associations. Considering only pathogenic or presumed pathogenic mutations, as determined by the American College of Medical Genetics and Genomics guidelines, and using Bonferroni and Benjamini-Hochberg corrections for multiple comparisons, we found that CRC tumors from AA patients harbored significantly more mutations of phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) than those from CC patients. CRC tumors in AA patients also appeared to harbor more mutations of mitogen-activated protein kinase kinase 1 (MAP2K1/MEK1), MPL proto-oncogene (MPL), thrombo-poietin receptor, and neurofibromin 1 (NF1) than those from CC patients. In contrast, CRCs from AA patients were likely to carry fewer mutations of ataxia-telangiectasia mutated (ATM), as well as of proto-oncogene B-Raf (BRAF), including the V600E variant, than those from CC patients. Rates of immunohistochemical positivity for epidermal growth factor receptor (EGFR) and DNA topoisomerase 2-alpha (TOP2A) tended to be higher in CRCs from AA patients than in CC patients. In NSCLC adenocarcinoma, BRAF variants appeared to be more frequent in the AA than in the CC cohort, whereas in squamous cell lung carcinoma, programmed death-ligand 1 (PD-L1) expression tended to be lower in the AA than in CC group. Moreover, HGG tumors from AA patients showed a trend toward harboring more mutations of protein tyrosine phosphatase non-receptor 11 (PTPN11), than HGG tumors from the CC cohort. In contrast, mutations of phosphatase and tensin homolog (PTEN) and tumor protein 53 (TP53) appeared to be higher in HGG tumors in CC patients than in their AA counterparts. Our data revealed significant differences and trends in molecular signatures of the three cancer types in AA and CC cohorts. These findings imply that there may be differences in carcinogenesis between AA and CC patients and that race may be a factor that should be considered regarding cancer incidence and outcome.

• Publisher: International Institute of Anticancer Research
• Subject: Adenocarcinoma / Aged / Apoptosis / Ataxia / Ataxia telangiectasia / Ataxia telangiectasia mutated protein / Black or African American - genetics / Black or African American - statistics & numerical data / Brain tumors / Breast cancer / Carcinogenesis / Carcinogens / Cell death / Cell growth / Chi-square test / Cohort Studies / Colorectal carcinoma / Deoxyribonucleic acid / DNA / DNA Mutational Analysis / DNA topoisomerase / Epidermal growth factor receptors / Female / Genetic Heterogeneity / Genetics / Growth factors / Health care access / Health Status Disparities / Heterogeneity / High-Throughput Nucleotide Sequencing / Humans / Immunohistochemistry / Kinases / Lung cancer / Lung carcinoma / Male / MAP kinase / Metastasis / Middle Aged / Minority & ethnic groups / Molecular biology / Mortality / Mutation / Mutation, Missense / Neoplasms - ethnology / Neoplasms - genetics / Neoplasms - pathology / Neurofibromin 1 / Next-generation sequencing / Non-small cell lung carcinoma / Nucleotide sequence / Oncogenes / p53 Protein / Patients / PD-L1 protein / Phosphatase / Phosphatidylinositol 4,5-diphosphate / Protein-tyrosine-phosphatase / Proteins / Proto-Oncogene Mas / Race / Race factors / Racial Groups - genetics / Receptors / Squamous cell carcinoma / Tumors / Tyrosine / United States - epidemiology / White People - genetics / White People - statistics & numerical data