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Efficient separation of phosphopeptides employing a Ti/Nb-functionalized core-shell structure solid-phase extraction nanosphere
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Efficient separation of phosphopeptides employing a Ti/Nb-functionalized core-shell structure solid-phase extraction nanosphere
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Journal Article
Efficient separation of phosphopeptides employing a Ti/Nb-functionalized core-shell structure solid-phase extraction nanosphere
2021
Overview
A strategy for effectively enriching global phosphopeptides was successfully developed by using ammonia methyl phosphate (APA) as a novel chelating ligand and Ti
4+
and Nb
5+
as double functional ions (referred to as Fe
3
O
4
@mSiO
2
@APA@Ti
4+
/Nb
5+
). With the advantage of large specific surface area (151.1 m
2
/g), preeminent immobilized ability for metal ions (about 8% of total atoms), and unbiased enrichment towards phosphopeptides, Fe
3
O
4
@mSiO
2
@APA@Ti
4+
/Nb
5+
displays high selectivity (maximum mass ratio β-casein to BSA is 1:1500), low limit of detection (LOD, as low as 0.05 fmol), good relative standard deviation (RSD, lower than 7%), recovery rate of 87% (
18
O isotope labeling method), outstanding phosphopeptide loading capacity (330 μg/mg), and at least five times re-use abilities. In the examination of the actual sample, 24 phosphopeptides were successfully detected in saliva and 4 phosphopeptides were also selectively extracted from human serum. All experiments have shown that Fe
3
O
4
@mSiO
2
@APA@Ti
4+
/Nb
5+
exhibits exciting potential in view of the challenge of low abundance of phosphopeptides.
Graphical abstract
Publisher
Springer Vienna,Springer,Springer Nature B.V
Subject
