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Reassessment of the role of CaCO3 in n-butanol production from pretreated lignocellulosic biomass by Clostridium acetobutylicum
by
Mao, Guotao
, Su, Zengping
, Zhang, Zhanying
, Song, Andong
, Xie, Yaohuan
, Wang, Fengqin
, Zhang, Hongsen
, Xie, Hui
in
631/326/2522
/ 631/61/252
/ Amino acids
/ Biodegradation
/ Biomass
/ Butanol
/ Calcium
/ Calcium (extracellular)
/ Calcium (intracellular)
/ Calcium carbonate
/ Calcium influx
/ Clostridium acetobutylicum
/ Corn straw
/ Furans
/ Glycolysis
/ Humanities and Social Sciences
/ multidisciplinary
/ Organic acids
/ Pesticides
/ Phenols
/ Proteomics
/ Salts
/ Science
/ Science (multidisciplinary)
/ Sugar
2020
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Reassessment of the role of CaCO3 in n-butanol production from pretreated lignocellulosic biomass by Clostridium acetobutylicum
by
Mao, Guotao
, Su, Zengping
, Zhang, Zhanying
, Song, Andong
, Xie, Yaohuan
, Wang, Fengqin
, Zhang, Hongsen
, Xie, Hui
in
631/326/2522
/ 631/61/252
/ Amino acids
/ Biodegradation
/ Biomass
/ Butanol
/ Calcium
/ Calcium (extracellular)
/ Calcium (intracellular)
/ Calcium carbonate
/ Calcium influx
/ Clostridium acetobutylicum
/ Corn straw
/ Furans
/ Glycolysis
/ Humanities and Social Sciences
/ multidisciplinary
/ Organic acids
/ Pesticides
/ Phenols
/ Proteomics
/ Salts
/ Science
/ Science (multidisciplinary)
/ Sugar
2020
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Reassessment of the role of CaCO3 in n-butanol production from pretreated lignocellulosic biomass by Clostridium acetobutylicum
by
Mao, Guotao
, Su, Zengping
, Zhang, Zhanying
, Song, Andong
, Xie, Yaohuan
, Wang, Fengqin
, Zhang, Hongsen
, Xie, Hui
in
631/326/2522
/ 631/61/252
/ Amino acids
/ Biodegradation
/ Biomass
/ Butanol
/ Calcium
/ Calcium (extracellular)
/ Calcium (intracellular)
/ Calcium carbonate
/ Calcium influx
/ Clostridium acetobutylicum
/ Corn straw
/ Furans
/ Glycolysis
/ Humanities and Social Sciences
/ multidisciplinary
/ Organic acids
/ Pesticides
/ Phenols
/ Proteomics
/ Salts
/ Science
/ Science (multidisciplinary)
/ Sugar
2020
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Reassessment of the role of CaCO3 in n-butanol production from pretreated lignocellulosic biomass by Clostridium acetobutylicum
Journal Article
Reassessment of the role of CaCO3 in n-butanol production from pretreated lignocellulosic biomass by Clostridium acetobutylicum
2020
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Overview
In this study, the role of CaCO
3
in n-butanol production was further investigated using corn straw hydrolysate (CSH) media by
Clostridium acetobutylicum
CICC 8016. CaCO
3
addition stimulated sugars utilization and butanol production. Further study showed that calcium salts addition to CSH media led to the increase in Ca
2+
concentration both intracellularly and extracellularly. Interestingly, without calcium salts addition, intracellular Ca
2+
concentration in the synthetic P2 medium was much higher than that in the CSH medium despite the lower extracellular Ca
2+
concentrations in the P2 medium. These results indicated that without additional calcium salts, Ca
2+
uptake by
C. acetobutylicum
CICC 8016 in the CSH medium may be inhibited by non-sugar biomass degradation compounds, such as furans, phenolics and organic acids. Comparative proteomics analysis results showed that most enzymes involved in glycolysis, redox balance and amino acids metabolism were up-regulated with CaCO
3
addition. This study provides further insights into the role of CaCO
3
in n-butanol production using real biomass hydrolysate.
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