Journal Article

Development of Dermestes tessellatocollis Motschulsky under different constant temperatures and its implication in forensic entomology

Luo, Chengliang, Wang, Man, Wang, Yu, Liu, Niu, Chen, Rufeng, Xia, Shuixiu, Ren, Xinru, Wang, Jiangfeng, Xiu, Xiashui, Xu, Wang, Wang, Yinghui, Wang, Yuji, Hu, Guoliang, Zhu, Rui

2021

Overview

•Dermestes tessellatocollis is a forensically important insect for PMI estimation.•Development of D. tessellatocollis was monitored from 16 to 34 °C.•Development models were constructed which can be used to estimate PMImin.•Developmental threshold temperature and thermal constant were obtained.•Instar determination accuracy was unsatisfactory for application in casework. Dermestidae generally appears on dry corpses and carcasses, especially if mummified or skeletonized. They are forensically important insect species for estimating longer postmortem intervals (PMI). As they develop, Dermestidae larvae undergo multiple larval ecdyses; however, a lack of guidelines for determining the larval instar limits their forensic application. Herein, we explored how temperature impacts the development of Dermestes tessellatocollis Motschulsky, 1860 (Coleoptera: Dermestidae). At seven constant temperatures (16, 19, 22, 25, 28, 31, and 34 °C), the developmental time from egg to adult was 163.87 ± 9.19, 103.56 ± 3.02, 63.59 ± 2.88, 51.49 ± 2.74, 47.86 ± 3.01, 44.62 ± 4.65, and 41.80 ± 4.87 days respectively. Four morphological indexes, including head capsule width, pronotum width, mesonotum width, and body length, were taken in vivo at regular intervals to identify methods for larval instar determination in D. tessellatocollis. The acquired morphological data were used to simulate fitted curves and equations depicting the relationship between the four morphological indexes and instars. From the validation experiment, we could hardly determine a specific instar based on the morphological indexes. The combination of morphometric data (head capsule, pronotum, and mesonotum width) generated the classification accuracy at 100%, 87.5%, 85%, and 93% for the 1st, 2nd/3rd, 4th/5th, and 6th/7th instars, respectively. Nevertheless, the accuracy was unsatisfactory for application in forensic casework. This study provides fundamental development data for adopting D. tessellatocollis in minimum postmortem interval (PMImin) estimations; however, further studies are needed to improve the classification accuracy for the larval instar determination.

• Publisher: Elsevier B.V,Elsevier Limited
• Subject: adults / Animals / Body length / Classification / Coleoptera - growth & development / Data acquisition / Dermestes / Dermestidae / Developmental model / Eggs / Entomology / Forensic Entomology / Forensic science / Forensic sciences / head / Humidity / In vivo measurements / In vivo methods and tests / Insects / Instar determination / Instars / Intervals / Laboratories / Larva - growth & development / Larvae / Minimum postmortem interval / Morphological indexes / Morphology / morphometry / Museums / postmortem changes / Pupa - growth & development / Regression analysis / Temperature / Temperature effects