Asset Details
Do you wish to reserve the book?
Gamma irradiation effects on in vitro shoot cultures of Dianthus caryophyllus L. and molecular characterization of mutants using ISSR markers
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Gamma irradiation effects on in vitro shoot cultures of Dianthus caryophyllus L. and molecular characterization of mutants using ISSR markers
Do you wish to request the book?
Gamma irradiation effects on in vitro shoot cultures of Dianthus caryophyllus L. and molecular characterization of mutants using ISSR markers
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Gamma irradiation effects on in vitro shoot cultures of Dianthus caryophyllus L. and molecular characterization of mutants using ISSR markers
2026
Overview
The current investigation was carried out to study the effect of different doses of gamma irradiation on
in vitro
shoot cultures of carnation (
Dianthus caryophyllus
L.) and characterization of mutants using ISSR markers. The trial was undertaken at the experimental farm and laboratory of the Department of Floriculture and Landscaping, Dr. YS Parmar University of Horticulture and Forestry, Nauni, Solan (HP). The experiment on ten carnation genotypes i.e. 7 mutants, 3 parents viz.; ‘Dark Rendezvous’, ‘Madras’ and ‘Tempo’ was laid out in Randomized Block Design (RBD) under polyhouse conditions. All the mutants developed after the irradiation with gamma rays showed variation in color. In this study, different morphological parameters of the mutants and their parents were recorded. Among different mutants, maximum plant height was recorded in mutant of ‘Dark Rendezvous’ i.e. ‘Rendez-Vous A
2
’ (67.70 cm) and maximum flower size in ‘Rendez-Vous C
2
’ (8.40 cm). Duration of flowering was recorded maximum in ‘Tempo A
1
’ (67.67 days) and vase life in ‘Rendez-Vous C
1
’ (14.79 days). For the majority of the characters analyzed, all mutants performed better in comparison to parents. Moreover, for the characterization of mutants at the molecular level, ten ISSR primers were used and DNA polymorphism was observed with four primers. A total of fifteen polymorphic bands were produced by these four primers. No monomorphic band was observed with any primer resulting in 100% polymorphism. Variations in the plant material can be explained through genetic polymorphism. These results directly strengthen the significance of mutation breeding in developing improved carnation cultivars and substantiate the use of molecular characterization for validating genetic variation. Furthermore, they emphasize the scope for the commercial expansion of carnation cultivation through the selection of promising mutants.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
