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Dmy initiates masculinity by altering Gsdf/Sox9a2/Rspo1 expression in medaka (Oryzias latipes)
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Dmy initiates masculinity by altering Gsdf/Sox9a2/Rspo1 expression in medaka (Oryzias latipes)
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Journal Article
Dmy initiates masculinity by altering Gsdf/Sox9a2/Rspo1 expression in medaka (Oryzias latipes)
2016
Overview
Despite identification of several sex-determining genes in non-mammalian vertebrates, their detailed molecular cascades of sex determination/differentiation are not known. Here, we used a novel RNAi to characterise the molecular mechanism of
Dmy
(the sex-determining gene of medaka)-mediated masculinity in XY fish.
Dmy
knockdown (
Dmy
-KD) suppressed male pathway (
Gsdf, Sox9a2
, etc.) and favoured female cascade (
Rspo1
, etc.) in embryonic XY gonads, resulting in a fertile male-to-female sex-reversal.
Gsdf, Sox9a2
and
Rspo1
directly interacted with
Dmy
and co-injection of
Gsdf
and
Sox9a2
re-established masculinity in XY-
Dmy
-KD transgenics, insinuating that
Dmy
initiates masculinity by stimulating and suppressing
Gsdf
/
Sox9a2
and
Rspo1
expression, respectively. Gonadal expression of
Wt1a
starts prior to
Dmy
and didn’t change upon
Dmy
-KD. Furthermore, Wt1a stimulated the promoter activity of
Dmy
, suggesting Wt1a as a regulator of
Dmy
. These findings provide new insights into the role of vertebrate sex-determining genes associated with the molecular interplay between the male and female pathways.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 38/15
/ 38/32
/ 38/39
/ 38/89
/ 42
/ 64
/ Amino Acid Sequence - genetics
/ Animals
/ Animals, Genetically Modified
/ Embryos
/ Female
/ Fish Proteins - biosynthesis
/ Gene Expression Regulation, Developmental
/ Gonads
/ Gonads - growth & development
/ Humanities and Social Sciences
/ Male
/ Mutation
/ Oryzias - growth & development
/ Science
/ Sex Determination Processes - genetics
/ SOX9 Transcription Factor - genetics
