Journal Article

Primary tumor associated macrophages activate programs of invasion and dormancy in disseminating tumor cells

Sharma, Ved P., Friedman, Madeline, Entenberg, David, Ye, Xianjun, Aguirre-Ghiso, Julio A., Coste, Anouchka, Lin, Yu, Karagiannis, George S., Duran, Camille L., Chen, Xiaoming, Wang, Yarong, Dalla, Erica, Oktay, Maja H., Traub, Brian, Singh, Deepak K., Borriello, Lucia, Sun, Dan, Condeelis, John S., Sosa, Maria Soledad

2022

Overview

Metastases are initiated by disseminated tumor cells (DTCs) that colonize distant organs. Growing evidence suggests that the microenvironment of the primary tumor primes DTCs for dormant or proliferative fates. However, the manner in which this occurs remains poorly understood. Here, using the Window for High-Resolution Intravital Imaging of the Lung (WHRIL), we study the live lung longitudinally and follow the fate of individual DTCs that spontaneously disseminate from orthotopic breast tumors. We find that spontaneously DTCs have increased levels of retention, increased speed of extravasation, and greater survival after extravasation, compared to experimentally metastasized tumor cells. Detailed analysis reveals that a subset of macrophages within the primary tumor induces a pro-dissemination and pro-dormancy DTC phenotype. Our work provides insight into how specific primary tumor microenvironments prime a subpopulation of cells for expression of proteins associated with dissemination and dormancy. The understanding of the mechanisms underlying the ability of disseminated tumor cells (DTCs) to form metastasis is incomplete. Here, by using high-resolution intravital imaging of the murine lung to track the fate of breast-derived DTCs, the authors show that macrophages within the primary tumor induce a pro-dissemination and pro-dormancy phenotype in tumor cells, favouring their extravasation in the lung.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 14/1 / 14/35 / 14/69 / 631/67/1347 / 631/67/327 / 631/67/71 / 96 / 96/100 / 96/106 / 96/109 / 96/35 / Animals / Breast cancer / Breast Neoplasms - genetics / Cancer / Cell Line, Tumor / Dormancy / Extravasation / Female / Gene expression / High resolution / Humanities and Social Sciences / Humans / Image resolution / Lungs / Macrophages / Medical schools / Medicine / Metastases / Metastasis / Mice / Mice, Inbred C57BL / Microenvironments / multidisciplinary / Neoplasms, Experimental / Neoplastic Stem Cells / Organs / Phenotype / Phenotypes / Science / Science (multidisciplinary) / Tumor cells / Tumor microenvironment / Tumor Microenvironment - physiology / Tumor-Associated Macrophages - physiology / Tumors / Visualization