Journal Article

Maltotriose-based probes for fluorescence and photoacoustic imaging of bacterial infections

Steinberg, Idan, Gowrishankar, Gayatri, Haywood, Tom, Zlitni, Aimen, Sam Gambhir, Sanjiv

2020

Overview

Currently, there are no non-invasive tools to accurately diagnose wound and surgical site infections before they become systemic or cause significant anatomical damage. Fluorescence and photoacoustic imaging are cost-effective imaging modalities that can be used to noninvasively diagnose bacterial infections when paired with a molecularly targeted infection imaging agent. Here, we develop a fluorescent derivative of maltotriose (Cy7-1-maltotriose), which is shown to be taken up in a variety of gram-positive and gram-negative bacterial strains in vitro. In vivo fluorescence and photoacoustic imaging studies highlight the ability of this probe to detect infection, assess infection burden, and visualize the effectiveness of antibiotic treatment in E. coli -induced myositis and a clinically relevant S. aureus wound infection murine model. In addition, we show that maltotriose is an ideal scaffold for infection imaging agents encompassing better pharmacokinetic properties and in vivo stability than other maltodextrins (e.g. maltohexose). Sensitive diagnostic tools for bacterial infections of wounds and surgical sites are necessary to enable early detection and determine optimal means of treatment. Here, the authors develop a fluorescent and optoacoustic probe based on a maltotriose scaffold, which is selectively taken up by gram-positive and gram-negative bacteria.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 14/34 / 59/5 / 631/1647/245/2160 / 631/92/613 / 64/60 / 692/699/255/1318 / 82/16 / Animal models / Animals / Antibiotics / Bacteria / Bacterial diseases / Bacterial infections / Carbocyanines - administration & dosage / Carbocyanines - chemistry / Diagnostic software / Diagnostic systems / Disease Models, Animal / Drug Stability / E coli / Escherichia coli - isolation & purification / Escherichia coli - metabolism / Female / Fluorescence / Fluorescent Dyes - administration & dosage / Fluorescent Dyes - chemistry / Fluorescent Dyes - metabolism / Fluorescent indicators / Gram-negative bacteria / Humanities and Social Sciences / Humans / Imaging / In vivo methods and tests / Infections / Injections, Intravenous / Luminescent Measurements - methods / Maltotriose / Mice / Microscopy, Fluorescence - methods / Molecular Imaging - methods / Molecular Probes - administration & dosage / Molecular Probes - chemistry / Molecular Probes - metabolism / multidisciplinary / Myositis / Myositis - diagnostic imaging / Myositis - microbiology / Pharmacokinetics / Photoacoustic Techniques - methods / Rats / Scaffolds / Science / Science (multidisciplinary) / Staphylococcus aureus - isolation & purification / Staphylococcus aureus - metabolism / Surgical site infections / Surgical Wound Infection - diagnostic imaging / Surgical Wound Infection - microbiology / Trisaccharides - administration & dosage / Trisaccharides - chemistry / Trisaccharides - metabolism / Wound infection