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Observing astrocyte polarization in brains from mouse chronically infected with Toxoplasma gondii
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Observing astrocyte polarization in brains from mouse chronically infected with Toxoplasma gondii
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Journal Article
Observing astrocyte polarization in brains from mouse chronically infected with Toxoplasma gondii
2024
Overview
Toxoplasma gondii
(
T. gondii
) is a protozoan parasite that infects approximately one-third of the global human population, often leading to chronic infection. While acute
T. gondii
infection can cause neural damage in the central nervous system and result in toxoplasmic encephalitis, the consequences of
T. gondii
chronic infection (TCI) are generally asymptomatic. However, emerging evidence suggests that TCI may be linked to behavioral changes or mental disorders in hosts. Astrocyte polarization, particularly the A1 subtype associated with neuronal apoptosis, has been identified in various neurodegenerative diseases. Nevertheless, the role of astrocyte polarization in TCI still needs to be better understood. This study aimed to establish a mouse model of chronic TCI and examine the transcription and expression levels of glial fibrillary acidic protein (GFAP), C3, C1q, IL-1α, and TNF-α in the brain tissues of the mice. Quantitative real-time PCR (qRT-PCR), enzyme-linked immunosorbent assay, and Western blotting were employed to assess these levels. Additionally, the expression level of the A1 astrocyte-specific marker C3 was evaluated using indirect fluorescent assay (IFA). In mice with TCI, the transcriptional and expression levels of the inflammatory factors C1q, IL-1α, and TNF-α followed an up-down-up pattern, although they remained elevated compared to the control group. These findings suggest a potential association between astrocyte polarization towards the A1 subtype and synchronized changes in these three inflammatory mediators. Furthermore, immunofluorescence assay (IFA) revealed a significant increase in the A1 astrocytes (GFAP
+
C3
+
) proportion in TCI mice. This study provides evidence that TCI can induce astrocyte polarization, a biological process that may be influenced by changes in the levels of three inflammatory factors: C1q, IL-1α, and TNF-α. Additionally, the release of neurotoxic substances by A1 astrocytes may be associated with the development of TCI.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Animals
/ Enzyme-linked immunosorbent assay
/ Female
/ Glial fibrillary acidic protein
/ Glial Fibrillary Acidic Protein - genetics
/ Glial Fibrillary Acidic Protein - metabolism
/ Humanities and Social Sciences
/ Mice
/ Science
/ Toxoplasmosis - parasitology
/ Toxoplasmosis, Cerebral - metabolism
/ Toxoplasmosis, Cerebral - parasitology
/ Toxoplasmosis, Cerebral - pathology
