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The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis
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The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis
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Journal Article
The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis
2021
Overview
The human orthologue of the tumor suppressor protein FBW7 is encoded by the Drosophila
archipelago (ago)
gene. Ago is an F-box protein that gives substrate specificity to its SCF ubiquitin ligase complex. It has a central role in multiple biological processes in a tissue-specific manner such as cell proliferation, cellular differentiation, hypoxia-induced gene expression. Here we present a previously unknown tissue-specific role of Ago in spermatid differentiation. We identified a classical mutant of
ago
which is semi-lethal and male-sterile. During the characterization of
ago
function in testis, we found that
ago
plays role in spermatid development, following meiosis. We confirmed spermatogenesis defects by silencing
ago
by RNAi in testes. The
ago
mutants show multiple abnormalities in elongating and elongated spermatids, including aberration of the cyst morphology, malformed mitochondrial structures, and individualization defects. Additionally, we determined the subcellular localization of Ago protein with mCherry-Ago transgene in spermatids. Our findings highlight the potential roles of Ago in different cellular processes of spermatogenesis, like spermatid individualization, and regulation of mitochondrial morphology.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Animals
/ Cell Differentiation - genetics
/ Drosophila melanogaster - genetics
/ Drosophila melanogaster - metabolism
/ Drosophila Proteins - genetics
/ Drosophila Proteins - metabolism
/ Humanities and Social Sciences
/ Hypoxia
/ Infertility, Male - genetics
/ Insects
/ Male
/ Meiosis
/ Mutants
/ Mutation
/ Proteins
/ Science
/ Testes
