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The type IV pili component PilO is a virulence determinant of Francisella novicida
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The type IV pili component PilO is a virulence determinant of Francisella novicida
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Journal Article
The type IV pili component PilO is a virulence determinant of Francisella novicida
2022
Overview
Francisella tularensis
is a highly pathogenic intracellular bacterium that causes the disease tularemia. While its ability to replicate within cells has been studied in much detail, the bacterium also encodes a less characterised type 4 pili (T4P) system. T4Ps are dynamic adhesive organelles identified as major virulence determinants in many human pathogens. In
F
.
tularensis
, the T4P is required for adherence to the host cell, as well as for protein secretion. Several components, including pilins, a pili peptidase, a secretin pore and two ATPases, are required to assemble a functional T4P, and these are encoded within distinct clusters on the
Francisella
chromosome. While some of these components have been functionally characterised, the role of PilO, if any, still is unknown. Here, we examined the role of PilO in the pathogenesis of
F
.
novicida
. Our results show that the PilO is essential for pilus assembly on the bacterial surface. In addition, PilO is important for adherence of
F
.
novicida
to human monocyte-derived macrophages, secretion of effector proteins and intracellular replication. Importantly, the
pilO
mutant is attenuated for virulence in BALB/c mice regardless of the route of infection. Following intratracheal and intradermal infection, the mutant caused no histopathology changes, and demonstrated impaired phagosomal escape and replication within lung liver as well as spleen. Thus, PilO is an essential virulence determinant of
F
.
novicida
.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Adhesion
/ Animals
/ Bacteria
/ Bacterial Adhesion - genetics
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Fimbriae, Bacterial - genetics
/ Fimbriae, Bacterial - metabolism
/ Fimbriae, Bacterial - ultrastructure
/ Francisella - ultrastructure
/ Francisella tularensis - genetics
/ Francisella tularensis - metabolism
/ Francisella tularensis - pathogenicity
/ Francisella tularensis - ultrastructure
/ Humans
/ Medicine
/ Medicine and Health Sciences
/ Mice
/ Mutants
/ Pili
/ Proteins
/ Research and Analysis Methods
/ Secretin
/ Spleen
/ Trachea
