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Presynaptic CLC-3 determines quantal size of inhibitory transmission in the hippocampus
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Presynaptic CLC-3 determines quantal size of inhibitory transmission in the hippocampus
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Journal Article
Presynaptic CLC-3 determines quantal size of inhibitory transmission in the hippocampus
2011
Overview
The authors report that the chloride channel CLC-3 colocalizes with the vesicular GABA transporter VGAT in the CA1 region of the hippocampus, where it affected Cl
−
-induced acidification of synaptic vesicles.
Clcn3
−/−
animals showed a decrement in inhibitory transmission, suggesting a decrease in neurotransmitter loading of synaptic vesicles.
The absence of the chloride channel CLC-3 in
Clcn3
−/−
mice results in hippocampal degeneration with a distinct temporal-spatial sequence that resembles neuronal loss in temporal lobe epilepsy. We examined how the loss of CLC-3 might affect GABAergic synaptic transmission in the hippocampus. An electrophysiological study of synaptic function in hippocampal slices taken from
Clcn3
−/−
mice before the onset of neurodegeneration revealed a substantial decrease in the amplitude and frequency of miniature inhibitory postsynaptic currents compared with those in wild-type slices. We found that CLC-3 colocalized with the vesicular GABA transporter VGAT in the CA1 region of the hippocampus. Acidification of inhibitory synaptic vesicles induced by Cl
−
showed a marked dependence on CLC-3 expression. The decrease in inhibitory transmission in
Clcn3
−/−
mice suggests that the neurotransmitter loading of synaptic vesicles was reduced, which we attribute to defective vesicular acidification. Our observations extend the role of Cl
−
in inhibitory transmission from that of a postsynaptic permeant species to a presynaptic regulatory element.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Animal Genetics and Genomics
/ Animals
/ Biomedical and Life Sciences
/ CA1 Region, Hippocampal - metabolism
/ CA1 Region, Hippocampal - ultrastructure
/ Chloride Channels - deficiency
/ Chloride Channels - genetics
/ Chloride Channels - physiology
/ gamma-Aminobutyric Acid - physiology
/ Hippocampus - ultrastructure
/ Inhibitory Postsynaptic Potentials - genetics
/ Mice
/ Neural Inhibition - genetics
/ Neural Inhibition - physiology
/ Presynaptic Terminals - metabolism
/ Presynaptic Terminals - ultrastructure
/ Rats
/ Synaptic Transmission - genetics
/ Synaptic Vesicles - metabolism
/ Synaptic Vesicles - ultrastructure
/ Vesicular Inhibitory Amino Acid Transport Proteins - physiology
