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Transgenic expression of dentin phosphoprotein (DPP) partially rescued the dentin defects of DSPP-null mice
by
Liu, Peihong
, Xie, Xiaohua
, Zhang, Hua
, Liang, Tian
, Qin, Chunlin
, Lu, Yongbo
in
Biology and Life Sciences
/ Collagen
/ Collagen (type I)
/ Computed tomography
/ Defects
/ Dentin
/ Dentinogenesis
/ Dentinogenesis imperfecta
/ Dentistry
/ Dspp protein
/ Electron microscopy
/ Extracellular matrix
/ Fragmentation
/ Fragments
/ Gene mutation
/ Gene mutations
/ Health aspects
/ Histology
/ Hydroxyapatite
/ Immunohistochemistry
/ Labelling
/ Medicine and Health Sciences
/ Mice
/ Mineralization
/ Mutation
/ Phosphates
/ Phosphoproteins
/ Physical Sciences
/ Proteins
/ Radiography
/ Research and Analysis Methods
/ Scanning electron microscopy
/ Scanning microscopy
/ Transgenic mice
/ Tubules
/ X-ray radiography
2018
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Transgenic expression of dentin phosphoprotein (DPP) partially rescued the dentin defects of DSPP-null mice
by
Liu, Peihong
, Xie, Xiaohua
, Zhang, Hua
, Liang, Tian
, Qin, Chunlin
, Lu, Yongbo
in
Biology and Life Sciences
/ Collagen
/ Collagen (type I)
/ Computed tomography
/ Defects
/ Dentin
/ Dentinogenesis
/ Dentinogenesis imperfecta
/ Dentistry
/ Dspp protein
/ Electron microscopy
/ Extracellular matrix
/ Fragmentation
/ Fragments
/ Gene mutation
/ Gene mutations
/ Health aspects
/ Histology
/ Hydroxyapatite
/ Immunohistochemistry
/ Labelling
/ Medicine and Health Sciences
/ Mice
/ Mineralization
/ Mutation
/ Phosphates
/ Phosphoproteins
/ Physical Sciences
/ Proteins
/ Radiography
/ Research and Analysis Methods
/ Scanning electron microscopy
/ Scanning microscopy
/ Transgenic mice
/ Tubules
/ X-ray radiography
2018
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Transgenic expression of dentin phosphoprotein (DPP) partially rescued the dentin defects of DSPP-null mice
by
Liu, Peihong
, Xie, Xiaohua
, Zhang, Hua
, Liang, Tian
, Qin, Chunlin
, Lu, Yongbo
in
Biology and Life Sciences
/ Collagen
/ Collagen (type I)
/ Computed tomography
/ Defects
/ Dentin
/ Dentinogenesis
/ Dentinogenesis imperfecta
/ Dentistry
/ Dspp protein
/ Electron microscopy
/ Extracellular matrix
/ Fragmentation
/ Fragments
/ Gene mutation
/ Gene mutations
/ Health aspects
/ Histology
/ Hydroxyapatite
/ Immunohistochemistry
/ Labelling
/ Medicine and Health Sciences
/ Mice
/ Mineralization
/ Mutation
/ Phosphates
/ Phosphoproteins
/ Physical Sciences
/ Proteins
/ Radiography
/ Research and Analysis Methods
/ Scanning electron microscopy
/ Scanning microscopy
/ Transgenic mice
/ Tubules
/ X-ray radiography
2018
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Transgenic expression of dentin phosphoprotein (DPP) partially rescued the dentin defects of DSPP-null mice
Journal Article
Transgenic expression of dentin phosphoprotein (DPP) partially rescued the dentin defects of DSPP-null mice
2018
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Overview
Mutations in the dentin sialophosphoprotein (DSPP) gene cause dentinogenesis imperfecta. After synthesis, DSPP is proteolytically processed into NH2- and COOH-terminal fragments. The NH2-terminal fragment of DSPP is highly glycosylated but not phosphorylated, whereas the COOH-terminal fragment (named \"dentin phosphoprotein\" or \"DPP\") is highly phosphorylated but not glycosylated. These two fragments are believed to perform distinct roles in dentin formation. To analyze the functions of DPP in dentinogenesis, we created \"Dspp-/-;DPP Tg mice\", which expressed transgenic DPP driven by a Type I collagen promoter but lacked the endogenous Dspp gene. We characterized the dentin of the Dspp-/-;DPP Tg mice using X-ray radiography, histology, scanning electron microscopy, double fluorochrome labeling, immunohistochemistry and in situ hybridization. Micro-computed tomography analyses revealed that at postnatal 6 months, the transgenic expression of DPP increased the dentin thickness of the Dspp-null mice by 97.1% and restored the dentin material density by 29.5%. Histological analyses showed that the Dspp-null mice manifested an abnormal widening of the predentin while the predentin in Dspp-/-;DPP Tg mice was narrower than in the Dspp-null mice. Scanning electron microscopy analyses showed that the dentinal tubules in the Dspp-/-;DPP Tg mice were better organized than in the Dspp-null mice. The double fluorochrome labeling analyses demonstrated that the dentin mineral deposition rate in the Dspp-/-;DPP Tg mice was significantly improved compared to that in the Dspp-null mice. These findings indicate that the transgenic expression of DPP partially rescued the dentin defects of the DSPP-null mice, suggesting that DPP may promote dentin formation and that the coordinated actions between DPP and the NH2-terminal fragment of DSPP may be necessary for dentinogenesis.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
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