Journal Article

Immunohistochemical expression of PAX-8 in Sudanese patients diagnosed with malignant female reproductive tract tumors

Mohamed, Areeg M., Ali, Eman T., Siddig, Emmanuel E., Hassan, Lamis Ahmed, Shafig, Irene R., Mohamed, Nouh S., Muneer, Mohamed S., Ahmed, Ayman, Yosif, Abubaker Ahmed

2020

Overview

Objectives Paired box protein-8 (PAX-8) immunohistochemical expression can be used as a diagnostic marker for epithelial cells tumors. This study aimed at investigating the immunohistochemical expression of PAX-8 among Sudanese females diagnosed with cervical, endometrial, and ovarian cancers between December 2017 and May 2019 by studying their Formalin-fixed paraffin embedded blocks. Results Sixty patients diagnosed with female reproductive tract cancers were included who aged 58.7 ± 6.9 years (range, 43—71). Cervix was the most common cancer site in 51/60 (85%) patients. Regarding cancer stage, there was 17 (28%) and 14 (23%) of the study population had stage 3B and 2B, respectively. The histopathological diagnosis included 20 (44%), 13 (29%), and 12 (27%) poorly, moderately, and well differentiated cervical squamous cell carcinoma (SCC) as well as 11 (73%), 2 (13%), 1 (7%), and 1 (7%) endometrial adenocarcinoma, metastatic adenocarcinoma, endocervical adenocarcinoma, and ovarian mucinous cyst adenocarcinoma, respectively. PAX-8 was positively expressed in 9 endometrial adenocarcinoma, 1 endocervical adenocarcinoma and 1 ovarian mucinous cyst adenocarcinoma, 2 poorly, and 1 moderately differentiated SCC. All patients diagnosed with well differentiated SCC and metastatic adenocarcinoma showed no expression of PAX-8. A statistically significant was seen for PAX-8 expression and the different histopathological diagnosis, P value < 0.001.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Adenocarcinoma / Adenocarcinoma - diagnosis / Adenocarcinoma - genetics / Age groups / Aged / Antibodies / Biomedical and Life Sciences / Biomedicine / Cell differentiation / Cervical cancer / Cervix / Diagnosis / Diagnostic immunohistochemistry / Endometrial cancer / Endometrium / Epithelial cells / Female / Female genital cancer / Female reproductive cancer / Females / Genital Neoplasms, Female / Health aspects / Humans / Immunohistochemical expression / Immunohistochemistry / Life Sciences / Medicine/Public Health / Metastases / Metastasis / Methods / Middle Aged / Ovarian cancer / Ovarian Neoplasms - diagnosis / Paired box protein-8 / Paraffin / Population studies / Proteins / Reproductive system / Research Note / Risk factors / Squamous cell carcinoma / Statistical analysis / Testing / Transcription factors / Tumors / Uterine Cervical Neoplasms