Journal Article

Molecular characterization of breast cancer cell lines through multiple omic approaches

Ward, Alison K., Vizeacoumar, Franco J., McDonald, Megan, Napper, Scott, Smith, Shari E., Kendall, Stephanie, Vizeacoumar, Frederick S., Anderson, Deborah H., Mellor, Paul

2017

Overview

Background Breast cancer cell lines are frequently used as model systems to study the cellular properties and biology of breast cancer. Our objective was to characterize a large, commonly employed panel of breast cancer cell lines obtained from the American Type Culture Collection (ATCC 30-4500 K) to enable researchers to make more informed decisions in selecting cell lines for specific studies. Information about these cell lines was obtained from a wide variety of sources. In addition, new information about cellular pathways that are activated within each cell line was generated. Methods We determined key protein expression data using immunoblot analyses. In addition, two analyses on serum-starved cells were carried out to identify cellular proteins and pathways that are activated in these cells. These analyses were performed using a commercial PathScan array and a novel and more extensive phosphopeptide-based kinome analysis that queries 1290 phosphorylation events in major signaling pathways. Data about this panel of breast cancer cell lines was also accessed from several online sources, compiled and summarized for the following areas: molecular classification, mRNA expression, mutational status of key proteins and other possible cancer-associated mutations, and the tumorigenic and metastatic capacity in mouse xenograft models of breast cancer. Results The cell lines that were characterized included 10 estrogen receptor (ER)-positive, 12 human epidermal growth factor receptor 2 (HER2)-amplified and 18 triple negative breast cancer cell lines, in addition to 4 non-tumorigenic breast cell lines. Within each subtype, there was significant genetic heterogeneity that could impact both the selection of model cell lines and the interpretation of the results obtained. To capture the net activation of key signaling pathways as a result of these mutational combinations, profiled pathway activation status was examined. This provided further clarity for which cell lines were particularly deregulated in common or unique ways. Conclusions These two new kinase or “Kin-OMIC” analyses add another dimension of important data about these frequently used breast cancer cell lines. This will assist researchers in selecting the most appropriate cell lines to use for breast cancer studies and provide context for the interpretation of the emerging results.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: American Type Culture Collection / Analysis / Animal models / Animals / Biomarkers / Biomedical and Life Sciences / Biomedicine / Breast cancer / Breast cancer cell lines / Breast Neoplasms - genetics / Breast Neoplasms - metabolism / Cancer cells / Cancer Research / Cancer therapies / Cell culture / Cell cycle / Cell Line, Tumor / Cell Transformation, Neoplastic - genetics / Cell Transformation, Neoplastic - metabolism / Chemotherapy / Computational Biology - methods / Data processing / Databases, Genetic / Development and progression / Disease Models, Animal / DNA methylation / DNA Mutational Analysis / Epidermal growth factor / ErbB-2 protein / Estrogen receptors / Estrogens / Female / Gene expression / Gene Expression Profiling / Gene Expression Regulation, Neoplastic / Genetic aspects / Genomics / Genomics - methods / Heterografts / Humans / Immunoglobulins / Infrared imaging systems / Kinases / Metastases / Metastasis / Metastatic / Mice / Mutation / Mutations / Oncology / Phosphorylation / Physiological aspects / Protein expression / Proteins / Proteome / Proteomics / Proteomics - methods / Research Article / Signal Transduction / Signaling pathway activation / Surgical Oncology / Tumor cell lines / Tumorigenic / Xenografts