Journal Article

Inhibition effects of patchouli alcohol against influenza a virus through targeting cellular PI3K/Akt and ERK/MAPK signaling pathways

Zhang, Yang, Wang, Shuyao, Hao, Cui, Wang, Wei, Liu, Wei, Yu, Yunjia

2019

Overview

Background Patchouli alcohol (PA) is a tricyclic sesquiterpene extracted from Pogostemonis Herba , which is a traditional Chinese medicine used for therapy of inflammatory diseases. Recent studies have shown that PA has various pharmacological activities, including anti-bacterial and anti-viral effects. Methods In this study, the anti-influenza virus (IAV) activities and mechanisms were investigated both in vitro and in vivo. The inhibitory effects of PA against IAV in vitro were evaluated by plaque assay and immunofluorescence assay. The neuraminidase inhibition assay, hemagglutination inhibition (HI) assay, and western blot assay were used to explore the anti-viral mechanisms. The anti-IAV activities in vivo were determined by mice pneumonia model and HE staining. Results The results showed that PA significantly inhibited different IAV strains multiplication in vitro, and may block IAV infection through inactivating virus particles directly and interfering with some early stages after virus adsorption. Cellular PI3K/Akt and ERK/MAPK signaling pathways may be involved in the anti-IAV actions of PA. Intranasal administration of PA markedly improved mice survival and attenuated pneumonia symptoms in IAV infected mice, comparable to the effects of Oseltamivir. Conclusions Therefore, Patchouli alcohol has the potential to be developed into a novel anti-IAV agent in the future.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: 1-Phosphatidylinositol 3-kinase / Administration, Intranasal / Adsorption / AKT protein / Alcohol / alcohols / Animals / Antibacterial agents / Antiviral agents / Antiviral Agents - administration & dosage / Antiviral Agents - pharmacology / Antiviral effects / antiviral properties / Asian medicine / Biomedical and Life Sciences / Biomedicine / Cytotoxicity / Disease Models, Animal / Drug resistance / enzyme inhibition / Epidemics / ERK/MAPK pathway / Exo-a-sialidase / Extracellular signal-regulated kinase / Fluorescent antibody technique / Health aspects / hemagglutination / Hemagglutination inhibition / Immunofluorescence / Immunoglobulins / Infections / Inflammatory diseases / Influenza / Influenza A / Influenza A virus / Influenza A virus - growth & development / Influenza viruses / Intranasal administration / Lung - pathology / MAP kinase / Medical research / medicinal properties / Mice / mitogen-activated protein kinase / Novels / Oriental traditional medicine / Orthomyxoviridae Infections - drug therapy / Orthomyxoviridae Infections - pathology / Oseltamivir / Oseltamivir phosphate / Patchouli alcohol / Penicillin / Peramivir / phosphatidylinositol 3-kinase / PI3K/Akt pathway / Plaque assay / Pneumonia / Pogostemon / Pogostemon cablin / Proteins / Sesquiterpenes - administration & dosage / Sesquiterpenes - pharmacology / sesquiterpenoids / sialidase / Signal transduction / Signal Transduction - drug effects / staining / Survival Analysis / Swine flu / Traditional Chinese medicine / Treatment Outcome / virion / Virology / Viruses / Western blotting