Journal Article

Seroprevalence and associated risk factors for vector-borne pathogens in dogs from Egypt

Sazmand, Alireza, Selim, Abdelfattah, Alanazi, Abdullah D., Otranto, Domenico

2021

Overview

Background Dogs play an important role as reservoirs of many zoonotic vector-borne pathogens worldwide, yet reports of canine vector-borne diseases (CVBDs) in Egypt are scarce. Methods Serum samples were collected from pet dogs ( n  = 500) of the three most common breeds (German Shepherd, Rottweiler and Pit Bull) in five Governates of Cairo ( n  = 230), Giza ( n  = 110), Al-Qalyubia ( n  = 60), Al-Gharbia ( n  = 60) and Kafr El-Sheikh ( n  = 40) with a hot desert climate. The presence of antibodies to Anaplasma spp. ( A. phagocytophilum , A. platys ), Ehrlichia spp. ( E. canis , E. chaffeensis , E. ewingii ), Borrelia burgdorferi ( s.l. ) and Dirofilaria immitis were assessed using IDEXX SNAP ® 4Dx ® ELISA tests. For each pathogen, risk factors (i.e. geographical area, keeping condition, sex, age, breed, tick infestation, weekly sanitation of dog enclosures and application of ectoparasiticides) were evaluated by logistic regression approach. Results In total, 18.2% ( n  = 91, 95% CI 15.1–21.8) of dogs scored seropositive for at least one pathogen, the most frequent being Ehrlichia spp. ( n  = 56; 11.2%; 95% CI 8.7–14.3) followed by Anaplasma spp. ( n  = 33; 6.6%, 95% CI 4.7–9.1), Borrelia burgdorferi ( s.l. ) ( n  = 9; 1.8%, 95% CI 0.9–3.4) and D. immitis ( n  = 7; 1.4%, 95% CI 0.9–2.9). In the tested population, 15.4% (95% CI 12.5–18.8) of dogs were exposed to a single pathogen while 2.4 (95% CI 1.4–4.2) and 0.4% (95% CI 0.1–1.4) were simultaneously exposed to two or three pathogens, respectively. Major risk factors associated with VBDs were living outdoors ( Anaplasma spp., P  = 0.0001; Ehrlichia spp., P  = 0.0001), female sex ( Ehrlichia spp., P  = 0.005), German Shepherd breed ( Anaplasma spp., P  = 0.04; Ehrlichia spp., P  = 0.03), tick infestation ( Anaplasma spp., P  = 0.0001; Ehrlichia spp., P  = 0.0001; B. burgdorferi ( s.l. ), P  = 0.003; D. immitis, P  = 0.02), irregular sanitation ( Anaplasma spp., P  = 0.0001; Ehrlichia spp., P  = 0.0001; B. burgdorferi ( s.l. ), P  = 0.002; D. immitis, P  = 0.01) and not using ectoparasiticides ( Anaplasma spp., P  = 0.0001; Ehrlichia spp., P  = 0.0001; B. burgdorferi ( s.l. ), P  = 0.007). Conclusion To our knowledge, this is the first large-scale seroepidemiological study of CVBDs in Egypt. Considering that all of the detected pathogens are potentially zoonotic, effective ectoparasite control strategies, regular examination of pet dogs and successful chemoprophylaxis are advocated. Graphical Abstract

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Anaplasma / Animals / Anorexia / Antibodies / Antibodies, Bacterial - blood / Antibodies, Helminth - blood / Arachnids / Arid climates / arid zones / Arthropods / Asymptomatic / Bacteria - classification / Bacteria - genetics / Bacteria - immunology / Bacteria - pathogenicity / Biomedical and Life Sciences / Biomedicine / blood serum / Borrelia / Borrelia burgdorferi / Bull Terrier / Canine vector-borne pathogens / chemoprevention / Dirofilaria / Dirofilaria immitis / Disease Vectors / Dog Diseases - epidemiology / Dog Diseases - immunology / Dog Diseases - microbiology / Dog Diseases - parasitology / Dogs / Ectoparasites / ectoparasiticides / Egypt / Egypt - epidemiology / Ehrlichia / ELISA / Entomology / Female / females / Fever / German Shepherd / Health aspects / Health risks / Identification and classification / Infections / Infectious Diseases / Infestation / Lyme disease / Male / Parasites - classification / Parasites - genetics / Parasites - immunology / Parasites - pathogenicity / Parasites of veterinary importance / Parasitology / Pathogenic microorganisms / Pathogens / Peptides / Pets / Pets - blood / Pets - microbiology / Pets - parasitology / Proteins / Regression analysis / risk / Risk analysis / Risk Factors / Rottweiler / Sanitation / Seroepidemiologic Studies / Seroepidemiology / serological surveys / Serology / seroprevalence / Serum / Sex / Statistics / tick infestations / Tropical Medicine / Vector-borne diseases / Vectors (Biology) / Veterinary Medicine/Veterinary Science / Virology / Weekly / Zoonoses