Journal Article

Hindering the biofilm of microbial pathogens and cancer cell lines development using silver nanoparticles synthesized by epidermal mucus proteins from Clarias gariepinus

Ali, Ahmed O. M., Azab, Ahmad M., Alabssawy, Ahmed N., Ashry, Abdelrahman S., Khalaf-Allah, Hassan M. M., Abu-Elghait, Mohammed, Sabra, Abu-Bakr A. A., Salem, Salem S.

2024

Overview

Scientists know very little about the mechanisms underlying fish skin mucus, despite the fact that it is a component of the immune system. Fish skin mucus is an important component of defence against invasive infections. Recently, Fish skin and its mucus are gaining interest among immunologists. Characterization was done on the obtained silver nanoparticles Ag combined with Clarias gariepinus catfish epidermal mucus proteins (EMP-Ag-NPs) through UV–vis, FTIR, XRD, TEM, and SEM. Ag-NPs ranged in size from 4 to 20 nm, spherical in form and the angles were 38.10°, 44.20°, 64.40°, and 77.20°, Where wavelength change after formation of EMP-Ag-NPs as indicate of dark brown, the broad band recorded at wavelength at 391 nm. Additionally, the antimicrobial, antibiofilm and anticancer activities of EMP-Ag-NPs was assessed. The present results demonstrate high activity against unicellular fungi C. albicans , followed by E. faecalis . Antibiofilm results showed strong activity against both S. aureus and P. aeruginosa pathogens in a dose-dependent manner, without affecting planktonic cell growth. Also, cytotoxicity effect was investigated against normal cells (Vero), breast cancer cells (Mcf7) and hepatic carcinoma (HepG2) cell lines at concentrations (200–6.25 µg/mL) and current results showed highly anticancer effect of Ag-NPs at concentrations 100, 5 and 25 µg/mL exhibited rounding, shrinkage, deformation and granulation of Mcf7 and HepG2 with IC50 19.34 and 31.16 µg/mL respectively while Vero cells appeared rounded at concentration 50 µg/mL and normal shape at concentration 25, 12.5 and 6.25 µg/ml with IC50 35.85 µg/mL. This study evidence the potential efficacy of biologically generated Ag-NPs as a substitute medicinal agent against harmful microorganisms. Furthermore, it highlights their inhibitory effect on cancer cell lines.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: AgNPs / Analysis / Animals / Anti-Infective Agents - chemistry / Anti-Infective Agents - pharmacology / Antibiofilm / Antibiotics / Anticancer properties / Antimicrobial / Antimicrobial agents / antineoplastic activity / Antineoplastic Agents - chemistry / Antineoplastic Agents - pharmacology / Applied Microbiology / Bacteria / Bacterial infections / Biochemical Engineering / biofilm / Biofilms / Biofilms - drug effects / Biofilms - growth & development / Biomedical Engineering/Biotechnology / Biosynthesis / Biotechnology / Breast cancer / breast neoplasms / Cancer / Candida albicans - drug effects / carcinoma / catfish / Catfishes / cell growth / Cell Line, Tumor / Chemistry / Chemistry and Materials Science / Chlorocebus aethiops / Clarias gariepinus / Clarias gariepinus catfish / Cytotoxicity / deformation / dose response / Epidermis - metabolism / Fish Proteins - chemistry / Fish Proteins - metabolism / Fish Proteins - pharmacology / Fish skin / Fishes / Fungi / Genetic Engineering / Gram-positive bacteria / Granulation / Health aspects / Humans / Immune system / inhibitory concentration 50 / Liver cancer / Metal Nanoparticles - chemistry / Microbial mats / Microbial Sensitivity Tests / Microorganisms / Mucus / Mucus - metabolism / Nanoparticles / nanosilver / neoplasm cells / Pathogenic microorganisms / Pathogens / Physiological aspects / plankton / Plant Breeding/Biotechnology / Prevention / Proteins / Pseudomonas aeruginosa - drug effects / shrinkage / Silver / Silver - chemistry / Silver - pharmacology / Skin / Staphylococcus aureus - drug effects / Staphylococcus aureus - physiology / Tumor cell lines / Vero Cells / Wavelength / wavelengths