Journal Article

Integrated metabolome and transcriptome analysis of differences in quality of ripe Lycium barbarum L. fruits harvested at different periods

Yao, Wenkong, Liu, Deshuai, Wang, Ye, Yuan, Miao, Feng, Mei, Zhang, Li

2024

Overview

Background Wolfberry is well-known for its high nutritional value and medicinal benefits. Due to the continuous ripening nature of Goji berries and the fact that they can be commercially harvested within a few weeks, their phytochemical composition may change during the harvesting process at different periods. Result The involved molecular mechanisms of difference in fruit quality of ripe Lycium barbarum L. harvested at four different periods were investigated by transcriptomic and metabolomics analyses for the first time. According to the results we obtained, it was found that the appearance quality of L. barbarum fruits picked at the beginning of the harvesting season was superior, while the accumulation of sugar substances in L. barbarum fruits picked at the end of the harvesting season was better. At the same time the vitamin C and carotenoids content of wolfberry fruits picked during the summer harvesting season were richer. Ascorbic acid, succinic acid, glutamic acid, and phenolic acids have significant changes in transcription and metabolism levels. Through the network metabolic map, we found that ascorbic acid, glutamic acid, glutamine and related enzyme genes were differentially accumulated and expressed in wolfberry fruits at different harvesting periods. Nevertheless, these metabolites played important roles in the ascorbate–glutathione recycling system. Ascorbic acid, phenolic substances and the ascorbate–glutathione recycling system have antioxidant effects, which makes the L. barbarum fruits harvested in the summer more in line with market demand and health care concepts. Conclusion This study laid the foundation for understanding the molecular regulatory mechanisms of quality differences of ripe wolfberry fruits harvested at different periods, and provides a theoretical basis for enhancing the quality of L. barbarum fruits.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abscisic acid / Agriculture / Analysis / antioxidants / Ascorbate–glutathione recycling system / Ascorbic acid / Ascorbic Acid - metabolism / Berries / Biomedical and Life Sciences / Biotechnology / Carotenoids / Chemical composition / chemical constituents of plants / enzymes / Food quality / Fruit - metabolism / Fruit quality / Fruits / Gene expression / Gene Expression Profiling / Genetic aspects / Glutamates - metabolism / Glutamic acid / Glutamine / Glutathione / Glutathione - metabolism / Goji berries / Growth / health services / Life Sciences / Lycium / Lycium - genetics / Lycium - metabolism / Lycium barbarum / metabolism / Metabolites / Metabolome / Metabolomics / Molecular modelling / Nutritive value / Phenolic acids / Phenols / Picking time / Plant Sciences / Recycling / Recycling systems / Regulatory mechanisms (biology) / Ripening / Seasons / Software packages / Succinic acid / Sugar / Summer / supply balance / Sustainable Agriculture / Transcription factors / Transcriptomes / Transcriptomics / Tree Biology / Wolfberry