Journal Article

Isolation and cultivation of a novel sulfate-reducing magnetotactic bacterium belonging to the genus Desulfovibrio

Hideki Kobayashi, Akira Inoue, Toru Mizuki, Toru Maekawa, Hirokazu Shimoshige, Shigeru Shimamura

2021

Overview

Magnetotactic bacteria (MTB) synthesize magnetosomes composed of membrane-enveloped magnetite (Fe 3 O 4 ) and/or greigite (Fe 3 S 4 ) nanoparticles in the cells. It is known that the magnetotactic Deltaproteobacteria are ubiquitous and inhabit worldwide in the sediments of freshwater and marine environments. Mostly known MTB belonging to the Deltaproteobacteria are dissimilatory sulfate-reducing bacteria that biomineralize bullet-shaped magnetite nanoparticles, but only a few axenic cultures have been obtained so far. Here, we report the isolation, cultivation and characterization of a dissimilatory sulfate-reducing magnetotactic bacterium, which we designate “strain FSS-1”. We found that the strain FSS-1 is a strict anaerobe and uses casamino acids as electron donors and sulfate as an electron acceptor to reduce sulfate to hydrogen sulfide. The strain FSS-1 produced bullet-shaped magnetite nanoparticles in the cells and responded to external magnetic fields. On the basis of 16S rRNA gene sequence analysis, the strain FSS-1 is a member of the genus Desulfovibrio , showing a 96.7% sequence similarity to Desulfovibrio putealis strain B7-43 T . Futhermore, the magnetosome gene cluster of strain FSS-1 was different from that of Desulfovibrio magneticus strain RS-1. Thus, the strain FSS-1 is considered to be a novel sulfate-reducing magnetotactic bacterium belonging to the genus Desulfovibrio .

• Publisher: Public Library of Science (PLoS),Public Library of Science
• Subject: Amino acids / Analysis / Aquatic environment / Bacteria / Biology and Life Sciences / Cell culture / Cell size / Crystal morphology / Crystal structure / Crystals / Cytology / Desulfovibrio / Desulfovibrio desulfuricans / Earth Sciences / Electronics / Engineering and Technology / Ferrosoferric Oxide / Graduate schools / Graduate studies / Hydrogen / Iron oxides / Iron sulfides / Magnetite / Magnetite Nanoparticles / Marine technology / Medicine / Methodology / Morphology / Nanoparticles / Phylogenetics / Physical Sciences / Ponds / Prokaryotes / Q / R / Research and Analysis Methods / Research facilities / RNA, Bacterial / RNA, Ribosomal, 16S / Science / Science and technology / Sediments / Size distribution / Sulfate reduction / Sulfates / Tubes