Journal Article

Biotype Characterization, Developmental Profiling, Insecticide Response and Binding Property of Bemisia tabaci Chemosensory Proteins: Role of CSP in Insect Defense

Xuan, Ning, Rajashekar, Balaji, Arnaud, Philippe, Fan, Zhongxue, Ma, Hongmei, Xie, Hongyan, Liu, Guoxia, Guo, Xia, Picimbon, Jean-François, Offmann, Bernard

2016

Overview

Chemosensory proteins (CSPs) are believed to play a key role in the chemosensory process in insects. Sequencing genomic DNA and RNA encoding CSP1, CSP2 and CSP3 in the sweet potato whitefly Bemisia tabaci showed strong variation between B and Q biotypes. Analyzing CSP-RNA levels showed not only biotype, but also age and developmental stage-specific expression. Interestingly, applying neonicotinoid thiamethoxam insecticide using twenty-five different dose/time treatments in B and Q young adults showed that Bemisia CSP1, CSP2 and CSP3 were also differentially regulated over insecticide exposure. In our study one of the adult-specific gene (CSP1) was shown to be significantly up-regulated by the insecticide in Q, the most highly resistant form of B. tabaci. Correlatively, competitive binding assays using tryptophan fluorescence spectroscopy and molecular docking demonstrated that CSP1 protein preferentially bound to linoleic acid, while CSP2 and CSP3 proteins rather associated to another completely different type of chemical, i.e. α-pentyl-cinnamaldehyde (jasminaldehyde). This might indicate that some CSPs in whiteflies are crucial to facilitate the transport of fatty acids thus regulating some metabolic pathways of the insect immune response, while some others are tuned to much more volatile chemicals known not only for their pleasant odor scent, but also for their potent toxic insecticide activity.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acari / Acids / Acrolein - analogs & derivatives / Acrolein - metabolism / Adults / Agreements / Aleyrodidae / Amino Acid Sequence / Analysis / Animals / Bemisia / Bemisia tabaci / Binding / Biology and Life Sciences / Biotechnology / Biotypes / Butterflies & moths / Chemical properties / Chemoreception / Cinnamaldehyde / Clone Cells / CSP protein / CSP1 protein / Deoxyribonucleic acid / Developmental stages / DNA / DNA sequencing / Drosophila melanogaster / Expressed Sequence Tags / Fatty acids / Fluorescence / Fluorescence spectroscopy / Gene expression / Gene Expression Profiling / Gene Expression Regulation, Developmental - drug effects / Gene sequencing / Genetic aspects / Helicobacter pylori / Hemiptera / Hemiptera - drug effects / Hemiptera - genetics / Hemiptera - growth & development / Hemiptera - immunology / Immune response / Immune system / Insect Proteins - chemistry / Insect Proteins - genetics / Insect Proteins - metabolism / Insecticide resistance / Insecticides / Insecticides - toxicity / Insects / Ligands / Linoleic acid / Linoleic Acid - metabolism / Metabolic pathways / Mitochondrial DNA / Molecular docking / Molecular Docking Simulation / Myzus persicae / Neonicotinoids / Nitro Compounds - toxicity / Odor / Odors / Oxazines - toxicity / Pesticides / Phylogeny / Physiology / Potatoes / Proteins / Research and Analysis Methods / Ribonucleic acid / RNA / Spectroscopy / Studies / Sweet potatoes / Thiamethoxam / Thiazoles - toxicity / Time Factors / Tryptophan / Whiteflies / Young adults