Journal Article

Impact of TIEG1 Deletion on the Passive Mechanical Properties of Fast and Slow Twitch Skeletal Muscles in Female Mice

Pouletaut, Philippe, Vayssade, Muriel, Kammoun, Malek, Subramaniam, Malayannan, Hawse, John R., Bensamoun, Sabine F., Canon, Francis

2016

Overview

As transforming growth factor (TGF)-β inducible early gene-1 is highly expressed in skeletal muscle, the effect of TIEG1 gene deletion on the passive mechanical properties of slow and fast twitch muscle fibers was analyzed. Twenty five muscle fibers were harvested from soleus (Sol) and extensor digitorum longus (EDL) muscles from TIEG1-/- (N = 5) and control (N = 5) mice. Mechanical tests were performed on fibers and the dynamic and static stresses were measured. A viscoelastic Hill model of 3rd order was used to fit the experimental relaxation test data. In parallel, immunohistochemical analyses were performed on three serial transverse sections to detect the myosin isoforms within the slow and fast muscles. The percentage and the mean cross sectional area of each fiber type were calculated. These tests revealed a significant increase in the mechanical stress properties for the TIEG1-/- Sol fibers while a significant decrease appeared for the TIEG1-/- EDL fibers. Hill model tracked the shape of the experimental relaxation curve for both genotypes and both fiber types. Immunohistochemical results showed hypertrophy of all fiber types for TIEG1-/- muscles with an increase in the percentage of glycolytic fibers (IIX, and IIB) and a decrease of oxidative fibers (I, and IIA). This study has provided new insights into the role of TIEG1, known as KLF10, in the functional (SoltypeI: more resistant, EDLtypeIIB: less resistant) and morphological (glycolytic hypertrophy) properties of fast and slow twitch skeletal muscles. Further investigation at the cellular level will better reveal the role of the TIEG1 gene in skeletal muscle tissue.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animal biology / Animals / Biochemistry / Biochemistry, Molecular Biology / Bioengineering / Biology and Life Sciences / Biomechanical Phenomena / biomechanical phenomena/methods / Biomechanics / Clonal deletion / Computer Science / Data processing / DNA-Binding Proteins - genetics / Engineering Sciences / Female / Fibers / Gene Deletion / Genotypes / Glycerol / Glycolysis / Growth factors / Histology / Human health and pathology / Hypertrophy / Isoforms / Laboratories / Life Sciences / Mechanical properties / Mechanical tests / Mechanics / Medicine and Health Sciences / Mice / Modeling and Simulation / Models, Biological / Molecular biology / Muscle Fibers, Fast-Twitch - pathology / Muscle Fibers, Slow-Twitch - pathology / Muscle proteins / Muscle, Skeletal - pathology / Muscle, Skeletal - physiopathology / Muscles / Musculoskeletal system / Myosin / NMR / Nuclear magnetic resonance / Physiology / Research and Analysis Methods / Rodents / Skeletal muscle / Skeletal Muscle Myosins - metabolism / Stress, Mechanical / Tissues and Organs / Transcription Factors - genetics / Transforming growth factor / Transforming growth factors / Viscoelasticity