Journal Article

Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations

Eggan, Kevin, Avior, Yishai, Ghosh, Sulagna, Kashin, Seva, Kamitaki, Nolan, Ilic, Dusko, Genovese, Giulio, Merkle, Florian T., Charlton, Maura, Saphier, Genevieve, Mekhoubad, Shila, Mello, Curtis, Mitchell, Jana, Handsaker, Robert E., McCarroll, Steven A., Bar, Shiran, Benvenisty, Nissim

2017

Overview

The authors surveyed whole-exome and RNA-sequencing data from 252 unique pluripotent stem cell lines, some of which are in the pipeline for clinical use, and found that approximately 5% of cell lines had acquired mutations in the TP53 gene that allow mutant cells to rapidly outcompete non-mutant cells, but do not prevent differentiation. Expansion of human pluripotent stem cells carrying P53 mutations Copy number variants at particular genomic locations have been shown to arise in human pluripotent stem cells (hPSCs) under certain culture conditions, but the extent of acquired mutations in such culture remains to be determined. Kevin Eggan and colleagues surveyed the exomes of 140 human embryonic stem cell (hESC) lines, some of which are in the pipeline for clinical use.They identified mosaic mutations in the TP53 gene in a subset of cells for five unrelated hESC lines and show that the cells carrying the mutations outcompeted the non-mutant cells and could readily differentiate. Similar mutations were also identified by mining published datasets for an additional 14 hESC lines and more than 100 human induced PSC lines. The study highlights the need for in-depth characterization of cells derived from hPSCs before their use in the clinic. Human pluripotent stem cells (hPS cells) can self-renew indefinitely, making them an attractive source for regenerative therapies. This expansion potential has been linked with the acquisition of large copy number variants that provide mutated cells with a growth advantage in culture 1 , 2 , 3 . The nature, extent and functional effects of other acquired genome sequence mutations in cultured hPS cells are not known. Here we sequence the protein-coding genes (exomes) of 140 independent human embryonic stem cell (hES cell) lines, including 26 lines prepared for potential clinical use 4 . We then apply computational strategies for identifying mutations present in a subset of cells in each hES cell line 5 . Although such mosaic mutations were generally rare, we identified five unrelated hES cell lines that carried six mutations in the TP53 gene that encodes the tumour suppressor P53. The TP53 mutations we observed are dominant negative and are the mutations most commonly seen in human cancers. We found that the TP53 mutant allelic fraction increased with passage number under standard culture conditions, suggesting that the P53 mutations confer selective advantage. We then mined published RNA sequencing data from 117 hPS cell lines, and observed another nine TP53 mutations, all resulting in coding changes in the DNA-binding domain of P53. In three lines, the allelic fraction exceeded 50%, suggesting additional selective advantage resulting from the loss of heterozygosity at the TP53 locus. As the acquisition and expansion of cancer-associated mutations in hPS cells may go unnoticed during most applications, we suggest that careful genetic characterization of hPS cells and their differentiated derivatives be carried out before clinical use.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 631/532/2117 / 631/67 / Alleles / Amino acid sequence / Biotechnology / Cancer / Cell Count / Cell culture / Cell Differentiation - genetics / Cell Division - genetics / Cell Line / Computer applications / Copy number / Deoxyribonucleic acid / DNA / DNA - metabolism / DNA Mutational Analysis / Exome - genetics / Gene mutation / Gene sequencing / Genes, Dominant - genetics / Genes, p53 / Genetic aspects / Genomes / Good Manufacturing Practice / Health aspects / Heterozygosity / Human Embryonic Stem Cells - cytology / Human Embryonic Stem Cells - metabolism / Humanities and Social Sciences / Humans / Induced Pluripotent Stem Cells - cytology / Induced Pluripotent Stem Cells - metabolism / letter / Loss of heterozygosity / Loss of Heterozygosity - genetics / Medical research / Mosaicism / multidisciplinary / Mutation / Mutation - genetics / Neoplasms - genetics / Nucleotide sequence / p53 Protein / Physiological aspects / Pluripotency / Protein Domains / Ribonucleic acid / RNA / Science / Selection, Genetic / Stem cells / Tumor Suppressor Protein p53 - chemistry / Tumor Suppressor Protein p53 - genetics / Tumor Suppressor Protein p53 - metabolism / Tumors