Asset Details
Do you wish to reserve the book?
Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes
Do you wish to request the book?
Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes
2025
Overview
Mitochondria are central to cellular metabolism. Various fluorescence tools have been developed for imaging the mitochondrial environment. Yet, new reporters and imaging methods for directly reading the mitochondrial status are needed for high spatial-temporal resolution imaging. Here, we introduce PK Mito Deep Red (PKMDR), a low-phototoxicity mitochondrial probe for time-lapse imaging, whose fluorescence lifetime serves as a sensitive indicator of mitochondrial membrane potential (Δψ
m
). The positively charged PKMDR accumulates within mitochondria under a higher Δψ
m
, leading to concentration-induced quenching and a measurable decrease in fluorescence lifetime. Since mitochondrial respiration primarily regulates Δψ
m
, PKMDR’s fluorescence lifetime effectively reports on the status of oxidative phosphorylation. Using PKMDR with fluorescence lifetime imaging microscopy (FLIM), we visualize heterogeneous Δψ
m
across individual cells, organoids, and tissues over time. This method reliably reveals the heterogeneity between metabolically active peripheral mitochondria and relatively inactive perinuclear mitochondria in various cell types. Overall, PKMDR-FLIM is a robust tool for directly visualizing Δψ
m
with high spatiotemporal resolution.
Saimi and colleagues present PKMDR, a mitochondrial probe whose fluorescence lifetime serves as an indicator to monitor mitochondrial membrane potential and respiration dynamics with high resolution across cells and tissues.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
