Journal Article

Ultra Performance Liquid Chromatography-Based Metabonomic Study of Therapeutic Effect of the Surface Layer of Poria cocos on Adenine-Induced Chronic Kidney Disease Provides New Insight into Anti-Fibrosis Mechanism

Tan, Xiao-Jie, Zhao, Ying-Yong, Lin, Rui-Chao, Mei, Qibing, Feng, Ya-Long, Bai, Xu

2013

Overview

The surface layer of Poria cocos (Fu-Ling-Pi, FLP) is commonly used in traditional Chinese medicine and its diuretic effect was confirmed in rat. Ultra performance liquid chromatography/quadrupole time-of-flight high-sensitivity mass spectrometry and a novel mass spectrometry(Elevated Energy) data collection technique was employed to investigate metabonomic characteristics of chronic kidney disease (CKD) induced from adenine excess and the protective effects of FLP. Multiple metabolites are detected in the CKD and are correlated with progressive renal injury. Among these biomarkers, lysoPC(18∶0), tetracosahexaenoic acid, lysoPC(18∶2), creatinine, lysoPC (16∶0) and lysoPE(22∶0/0∶0) in the FLP-treated group were completely reversed to levels in the control group which lacked CKD. Combined with biochemistry and histopathology results, the changes in serum metabolites indicate that the perturbations of phospholipids metabolism, energy metabolism and amino acid metabolism are related to adenine-induced CKD and to the interventions of FLP on all the three metabolic pathways. FLP may regulate the metabolism of these biomarkers, especially their efficient utilization within the context of CKD. Furthermore, these biomarkers might serve as characteristics to explain the mechanisms of FLP.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acids / Adenine / Adenine - adverse effects / Amino acids / Animals / Biochemistry / Bioindicators / Biology / Biomarkers / Biomarkers - metabolism / Chinese medicine / Chromatography / Chromatography, High Pressure Liquid / Creatinine / Data collection / Diabetes / Edema / Energy metabolism / Ethanol / Fibrosis / Food / Herbal medicine / Histopathology / Kidney diseases / Kidneys / Laboratories / Life sciences / Liquid chromatography / Male / Mass spectrometry / Mass Spectrometry - methods / Mass spectroscopy / Medicine / Medicine, Chinese Traditional / Metabolic pathways / Metabolism / Metabolites / Metabolomics - methods / Multivariate analysis / NMR / Nuclear magnetic resonance / Oxidative stress / Phospholipids / Poria - chemistry / Quadrupoles / Rats / Rats, Sprague-Dawley / Renal Insufficiency, Chronic - chemically induced / Renal Insufficiency, Chronic - drug therapy / Renal Insufficiency, Chronic - metabolism / Renal Insufficiency, Chronic - pathology / Reproducibility of Results / Scientific imaging / Surface boundary layer / Surface layers / Traditional Chinese medicine