Journal Article

Diversity of Culicoides in the middle belt of Ghana with Implications on the transmission of Mansonella perstans; a molecular approach

Gawusu, Amidu, Arthur, Joseph F., Aniagyei, Wilfred, Adankwah, Ernest, Opoku, Vera Serwaa, Yeboah, Augustine, Acheampong, Isaac, Debrah, Linda Batsa, Wanji, Samuel, Jacobsen, Marc, Vivekanandan, Monika M., Lamptey, Millicent, Owusu, Dorcas O., Phillips, Richard O., Minadzi, Difery, Abass, Mohammed K., Debrah, Alexander Y.

2024

Overview

Background Culicoides , also known as biting midges, carry pathogens which include Mansonella perstans . Mansonella perstans is a nematode parasite implicated in a number of disease outcomes. Even though a high prevalence of about 75% M. perstans infection has been recorded in some communities in the middle belt of Ghana, and a wide diversity of Culicoides species has been identified, the exact Culicoides species transmitting M. perstans in Ghana has not yet been deciphered. This study therefore aimed at assessing the species diversity of Culicoides and their role in the transmission of M. perstans in the middle belt of Ghana. Methods Culicoides species were sampled from 11 communities in the Asante-Akim North and Sene West districts in the middle belt of Ghana. Centre for Disease Control (CDC) UV light traps, as well as human bait (i.e. human landing catch and engorged catch) methods were used to assess the species abundance and diversity of Culicoides in the study communities in the wet and dry season. A colorimetric Loop-Mediated Isothermal Amplification (LAMP) assay was performed to assess the vector competence of the various Culicoides species. Results A total of 4810 Culicoides from 6 species were sampled. These included Culicoides inornatipennis, C. milnei, C. schultzei, C. grahamii, C. neavei , and C. imicola. Culicoides imicola was the most abundant species (56%) followed by C. grahamii (16%). Light traps sampled the most diverse species (6 species). Human landing catch and engorged catch methods identified three anthropophilic species, C. grahamii, C. milnei , and C. inornatipennis , with C. grahamii being the most anthropophilic with a peak biting time between the hours of 5 p.m. to 6 p.m. Generally, there was relatively higher species abundance in the wet than dry season. LAMP assay identified C. grahamii as the potential vector for M. perstans transmission in the middle belt of Ghana. Conclusions For the first time, we have demonstrated that C. grahamii is the potential competent vector for M. perstans transmission in the middle belt of Ghana. It is more abundant in the rainy season and has a peak biting time between the hours of 5 and 6 p.m. Graphical Abstract

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abundance / Baits / Belts / Biodiversity / Biological diversity / Biomedical and Life Sciences / Biomedicine / Biting / Collectors / Colorimetry / Culicoides / Culicoides grahamii / Culicoides imicola / Culicoides species / Diptera / Disease control / Disease transmission / Distribution / Dominant species / Dry season / Entomology / Environmental aspects / Gene amplification / Ghana / Health aspects / humans / Identification / Infection / Infections / Infectious Diseases / Insects as carriers of disease / Landing / Light traps / loop-mediated isothermal amplification / Mansonella / Mansonella perstans / Medical laboratories / Microfilariae / Morphology / Nematoda / Parasites / Parasitological research / Parasitology / Pathogens / Rainy season / Risk factors / Roundworm infections / Seasons / species / species abundance / Species diversity / Tropical Medicine / Ultraviolet radiation / vector competence / Veterinary Medicine/Veterinary Science / Virology / Wet season