Journal Article

MiR-145 Inhibits Metastasis by Targeting Fascin Actin-Bundling Protein 1 in Nasopharyngeal Carcinoma

He, Qing-Mei, Liu, Na, Wen, Xin, Li, Ying-Qin, Yang, Xiao-Jing, Ren, Xian-Yue, Tang, Xin-Ran, Xu, Ya-Fei, Ma, Jun

2015

Overview

Based on our recent microarray analysis, we found that miR-145 was obviously downregulated in nasopharyngeal carcinoma (NPC) tissues. However, little is known about its function and mechanism involving in NPC development and progression. Quantitative RT-PCR was used to detect miR-145 expression in NPC cell lines and clinical samples. Wound healing, Transwell migration and invasion, three-dimension spheroid invasion assays, and lung metastasis model were performed to test the migratory, invasive, and metastatic ability of NPC cells. Luciferase reporter assay, quantitative RT-PCR, and Western blotting were used to verify the target of miR-145. MiR-145 was obviously decreased in NPC cell lines and clinical samples (P<0.01). Ectopic overexpression of miR-145 significantly inhibited the migratory and invasive ability of SUNE-1 and CNE-2 cells. In addition, stably overexpressing of miR-145 in SUNE-1 cells could remarkably restrain the formation of metastatic nodes in the lungs of mice. Furthermore, fascin actin-bundling protein 1 (FSCN1) was verified as a target of miR-145, and silencing FSCN1 with small RNA interfering RNA could suppress NPC cell migration and invasion. Our findings demonstrated that miR-145 function as a tumor suppressor in NPC development and progression via targeting FSCN1, which could sever as a potential novel therapeutic target for patients with NPC.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Actin / Analysis / Animals / Biotechnology / Bundling / Cancer metastasis / Carcinoma / Carcinoma - genetics / Carcinoma - metabolism / Carcinoma - pathology / Carrier Proteins - genetics / Carrier Proteins - metabolism / Cell adhesion & migration / Cell cycle / Cell growth / Cell Line, Tumor / Cell migration / Collaboration / DNA microarrays / Female / Gene expression / Gene Silencing / Health aspects / Humans / Invasiveness / Laboratories / Luciferase / Lungs / Medical prognosis / Medicine / Metastases / Metastasis / Mice / Mice, Inbred BALB C / Microfilament Proteins - genetics / Microfilament Proteins - metabolism / MicroRNAs / MicroRNAs - genetics / Model testing / Muscle proteins / Nasopharyngeal carcinoma / Nasopharyngeal Neoplasms - genetics / Nasopharyngeal Neoplasms - metabolism / Nasopharyngeal Neoplasms - pathology / Neoplasm Metastasis / Oncology / Polymerase chain reaction / Prostate cancer / Proteins / Ribonucleic acid / RNA / RNA-mediated interference / Therapeutic applications / Throat cancer / Tumor suppressor genes / Tumorigenesis / Tumors / Western blotting / Wound healing