Journal Article

In silico design of novel multi-epitope peptide vaccine against Neospora caninum induced cattle abortion targeting extracellular GRA2 and Nc-p43 protein

Sakib, Tasnim Sayem, Roy, Apurba, Hossain, Hemayet, Muktadir, Sofiur, Shishir, Tushar Ahmed, Hridy, Faria Khan, Akhand, Mst Rubaiat Nazneen, Ashraf, Tanvir, Belal, Anika Binte, Mashrur, Md. Nafij, Hasan, Md. Rakibul, Rahman, Md. Masudur, Rahman, Md. Mahfujur, Rani, Nurul Amin, Mohiuddin, A. S. M., Imranuzzaman, Md, Ahsan, Golam, Rahman, Mostafizor, Barua, Hridoy

2025

Overview

Neospora caninum (NC) is a protozoan infection causing neosporosis, a disease that leads to substantial economic loss in livestock, especially in cattle, sheep, and goats. Unfortunately, there is presently no viable vaccination or authorized therapy available. Our research applied a bioinformatics approach to design a multi-epitope peptide (MEP) vaccine aimed at the pathogenic proteins GRA2 and Nc-p43 of NC, which are critical in parasite-mediated antigenicity and host interactions. Consequently, our research employs an in-silico methodology, including protein sequence retrieval, epitope prediction, vaccine design, structural analysis, molecular docking, molecular dynamics simulations, immunological simulation, and codon optimization using in silico cloning. We conducted analyses of antigenicity, allergenicity, toxicity, topology, and immunogenicity using multiple bioinformatics methods and identified 4 CTL, 4 HTL, and 2 B-cell epitopes. The vaccine design was created by integrating an adjuvant and a PADRE sequence to enhance immunogenicity, along with linkers (AAY, GPGPG, KK) to facilitate appropriate structural assembly, which was then analyzed for optimal complete profiles. Molecular docking with the Bos taurus (cattle) TLR9 receptor demonstrated a robust binding affinity, scoring − 1183.4, while molecular dynamics (MD) simulations over 50 ns validated persistent interactions between the vaccine and immune receptors. Moreover, immunological models forecasted a robust adaptive immune response, marked by an increase in the production of cytokines and the establishment of memory T-lymphocytes. Ultimately, codon optimization and in silico cloning validated the capacity for effective expression in E. coli . The results demonstrate that the developed vaccine has considerable immunogenic potential against NC. Nonetheless, more in vitro and in vivo studies are necessary to confirm its efficiency.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/114 / 631/250 / 631/326 / 631/61 / Abortion / Adaptive immunity / Adjuvants / Algorithms / Allergenicity / Amino acid sequence / Animals / Antigenicity / Antigens / Antigens, Protozoan - immunology / Bioinformatics / Cattle / Cattle Diseases - immunology / Cattle Diseases - parasitology / Cattle Diseases - prevention & control / Cloning / Coccidiosis - immunology / Coccidiosis - parasitology / Coccidiosis - prevention & control / Coccidiosis - veterinary / Computer Simulation / Cytotoxicity / Design / E coli / Epitopes / Epitopes, B-Lymphocyte - immunology / Epitopes, T-Lymphocyte - immunology / Female / Flexibility / Humanities and Social Sciences / Immune response / Immunogenicity / In Silico study / Livestock / Lymphocytes / Lymphocytes B / Lymphocytes T / Memory cells / Molecular docking / Molecular Docking Simulation / Molecular dynamics / Molecular Dynamics Simulation / Multi-epitope peptide (MEP) vaccine / multidisciplinary / Neospora - immunology / Neospora caninum / Neosporosis / p43 Protein / Parasites / Peptides / Physicochemical properties / Protein Subunit Vaccines / Proteins / Protozoan Proteins - chemistry / Protozoan Proteins - genetics / Protozoan Proteins - immunology / Protozoan Vaccines - immunology / Science / Science (multidisciplinary) / Simulation / Structural analysis / TLR9 protein / Toll-like receptors / Topology / Toxicity / Vaccines / Vaccines, Subunit - immunology