Journal Article

Uncovering the molecular signature of feline diffuse iris melanoma through transcriptomic analysis of disease severity

Suárez-Bonnet, A., Pont, R. Tetas, Pittaway, R., Clark, R., Donnelly, K., Meynert, A., Becher, H., Fawkes, A., Blacklock, B., Blacklock, K. L. Bowlt, McGeachan, R., Kayes, D., Scurrell, E., Murphy, L., Fricker, G.

2025

Overview

Feline diffuse iris melanoma (FDIM) is the most common primary ocular tumour in cats, with metastatic disease occurring in 19–63% of patients. Greater intraocular invasion correlates with increased mortality. No effective therapeutics exist for metastatic FDIM, partly due to a lack of known molecular targets associated with aggressive tumour behaviour. Here, we define the transcriptomic landscape of FDIM in treatment-naïve cats using bulk RNA sequencing on laser capture microdissection and core biopsy specimens from formalin-fixed paraffin-embedded tissue. Samples included ‘iris melanosis’ (dysplastic melanocytes confined to the anterior iris; n = 7), ‘early FDIM’ (neoplastic melanocytes confined to the iris stroma; n = 13), and ‘late FDIM’ (neoplastic infiltration into the ciliary body and sclera; n = 13). Iris melanosis exhibited genetic overlap with early FDIM, supporting its reclassification as ‘melanoma in situ’. Early FDIM showed upregulation of genes linked to tumour initiation, immune recruitment, and motility (e.g., STOX1, PEG3, XIAP, MCAM, VIM ). Late FDIM exhibited immune microenvironment remodelling, immune evasion, and apoptosis inhibition (e.g., BIRC2, BIRC5, CCL2, HAVCR2 ), with downregulation of FOX1, FOXC2 , and SOX11 . These results provide critical biomarkers of disease severity, which may aid in the development of more accurate prognostic tests and more effective targeted therapies for FDIM.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/208/199 / 631/208/68 / 631/208/721 / 631/67/1484 / 631/67/1857 / 631/67/322 / 631/67/395 / 631/67/580 / 631/67/68 / 692/4028 / 692/699 / 692/699/3161 / Animals / Apoptosis / Biopsy / Cancer / Cat Diseases - genetics / Cat Diseases - pathology / Cats / Feline diffuse iris melanoma / Female / Gene expression / Gene Expression Profiling / Gene Expression Regulation, Neoplastic / Glaucoma / Humanities and Social Sciences / Iris / Iris Neoplasms - genetics / Iris Neoplasms - pathology / Iris Neoplasms - veterinary / Male / Melanocytes / Melanoma / Melanoma - genetics / Melanoma - metabolism / Melanoma - pathology / Melanoma - veterinary / Melanoma metastasis / Metastases / Metastasis / Microenvironments / Monocyte chemoattractant protein 1 / multidisciplinary / Peg3 protein / Science / Science (multidisciplinary) / Severity of Illness Index / Stroma / Survival analysis / Transcriptome / Transcriptomics / Tumors / Uveal melanoma / XIAP protein