Journal Article

The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors

Ferguson, Heather M., Saldarriaga, Segundo, Ortega-López, Leonardo D., Kohl, Alain, Betancourth, Mauro Pazmiño, Pondeville, Emilie, León, Renato, Almire, Floriane, Mirzai, Nosrat, Torres-Valencia, Sergio

2020

Overview

Background Entomological monitoring of Aedes vectors has largely relied on surveillance of larvae, pupae and non-host-seeking adults, which have been poorly correlated with human disease incidence. Exposure to mosquito-borne diseases can be more directly estimated using human landing catches (HLC), although this method is not recommended for Aedes- borne arboviruses. We evaluated a new method previously tested with malaria vectors, the mosquito electrocuting trap (MET) as an exposure-free alternative for measuring landing rates of Aedes mosquitoes on people. Aims were to (i) compare the MET to the BG-sentinel (BGS) trap gold standard approach for sampling host-seeking Aedes vectors; and (ii) characterize the diel activity of Aedes vectors and their association with microclimatic conditions. Methods The study was conducted over 12 days in Quinindé (Ecuador) in May 2017. Mosquito sampling stations were set up in the peridomestic area of four houses. On each day of sampling, each house was allocated either a MET or a BGS trap, which were rotated amongst the four houses daily in a Latin square design. Mosquito abundance and microclimatic conditions were recorded hourly at each sampling station between 7:00–19:00 h to assess variation between vector abundance, trapping methods, and environmental conditions. All Aedes aegypti females were tested for the presence of Zika (ZIKV), dengue (DENV) and chikungunya (CHIKV) viruses. Results A higher number of Ae. aegypti females were found in MET than in BGS collections, although no statistically significant differences in mean Ae. aegypti abundance between trapping methods were found. Both trapping methods indicated female Ae. aegypti had bimodal patterns of host-seeking, being highest during early morning and late afternoon hours. Mean Ae. aegypti daily abundance was negatively associated with daily temperature. No infection by ZIKV, DENV or CHIKV was detected in any Aedes mosquitoes caught by either trapping method. Conclusion We conclude the MET performs at least as well as the BGS standard and offers the additional advantage of direct measurement of per capita human-biting rates. If detection of arboviruses can be confirmed in MET-collected Aedes in future studies, this surveillance method could provide a valuable tool for surveillance and prediction on human arboviral exposure risk.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abundance / Adult / Aedes / Aedes - physiology / Aedes - virology / Aedes aegypti / Animals / Aquatic insects / Arbovirus / Arbovirus Infections - transmission / arboviruses / Biomedical and Life Sciences / Biomedicine / Biting / Chikungunya / Chikungunya Fever - transmission / Chikungunya virus / Chikungunya virus - isolation & purification / Collections / Comparative analysis / Culex - physiology / Culicidae / Dengue / Dengue - transmission / Dengue fever / Dengue Virus - isolation & purification / Diel activity / disease incidence / Diseases / Ecuador / Ecuador - epidemiology / Electrocutions / Entomology / Environmental conditions / environmental factors / Environmental quality / Exposure / Female / Females / Flavivirus - isolation & purification / Future predictions / Health aspects / Host-seeking / Houses / Housing / Human diseases / human-landing catches / Humans / imagos / Infectious Diseases / Insect Bites and Stings - epidemiology / insect vectors / Intelligence gathering / Landing / Larvae / Malaria / Male / Medical climatology / Medical research / Methodology / Methods / microclimate / Middle Aged / monitoring / Mosquito control / Mosquito Vectors - physiology / Mosquito Vectors - virology / mosquito-borne diseases / Mosquitoes / Observations / Parasitology / prediction / Prevention / Pupae / Residential areas / Sampling / Statistical analysis / Surveillance / temperature / Trapping / Tropical Medicine / Vector-borne diseases / Vectors / Veterinary Medicine/Veterinary Science / Viral diseases / Virology / Viruses / Zika / Zika Virus - isolation & purification / Zika Virus Infection - transmission